Antioxidant activities of ethanolic and acidic ethanolic extracts of astringent persimmon in H2O2-stimulated Caco-2 human colonic epithelial cells

• Published in: Food science and biotechnology Vol. 26; no. 4; pp. 1085 - 1091
• Main Authors: Kim, Leeseon, Kim, Yunyoung, Kwon, Oran, Kim, Ji Yeon
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.08.2017; Springer Nature B.V; 한국식품과학회
• Subjects: antioxidant activity; Antioxidants; Biological activity; Biological effects; Carbonyl compounds; Carbonyls; Catalase; Chemistry; Chemistry and Materials Science; Damage; Deoxyribonucleic acid; Diospyros kaki; DNA; DNA damage; Epithelial cells; Ethanol; Food Science; fruit extracts; Fruits; Glutathione; human cell lines; humans; Hydrogen peroxide; Normalizing; Nutrition; Oxidation; Oxidative stress; persimmons; protective effect; Superoxide dismutase; 식품과학; Caco-2 cells; Hydrogen peroxide; Tannin; Antioxidant; Astringent persimmon
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-017-0156-5

The aim of this study was to investigate the protective effects of ethanol (EAP) and acidic ethanol extracts (AEAP) of astringent persimmon fruits ( Diospyros kaki ) against hydrogen peroxide (H 2 O 2 )-induced oxidative stress in Caco-2 epithelial cells. Levels of reactive oxygen species (ROS) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein and DNA damage were measured. Treatment with H 2 O 2 induced oxidative stress in Caco-2 cells but had no effect on SOD activity. Both EAP and AEAP protected the cells against oxidative damage and exhibited similar effects on cellular ROS scavenging activity and protein carbonyl levels. However, AEAP was more potent than EAP in normalizing CAT activity and cytosolic GSH level, and in protecting against DNA damage. These results demonstrated that astringent persimmon fruit extracts were biologically active, and that the tannin-rich fraction (AEAP) possesses stronger protective activity against H 2 O 2 -induced oxidative stress in Caco-2 cells.