C9ORF72 repeat expansion causes vulnerability of motor neurons to Ca2+-permeable AMPA receptor-mediated excitotoxicity

• Published in: Nature communications Vol. 9; no. 1; pp. 1 - 14
• Main Authors: Selvaraj, Bhuvaneish T., Livesey, Matthew R., Zhao, Chen, Gregory, Jenna M., James, Owain T., Cleary, Elaine M., Chouhan, Amit K., Gane, Angus B., Perkins, Emma M., Dando, Owen, Lillico, Simon G., Lee, Youn-Bok, Nishimura, Agnes L., Poreci, Urjana, Thankamony, Sai, Pray, Meryll, Vasistha, Navneet A., Magnani, Dario, Borooah, Shyamanga, Burr, Karen, Story, David, McCampbell, Alexander, Shaw, Christopher E., Kind, Peter C., Aitman, Timothy J., Whitelaw, C. Bruce A., Wilmut, Ian, Smith, Colin, Miles, Gareth B., Hardingham, Giles E., Wyllie, David J. A., Chandran, Siddharthan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.01.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/100; 38/91; 42/41; 45; 631/378/1689/1285; 631/378/2632/1664; 9/74; Amyotrophic lateral sclerosis; Calcium; Calcium permeability; CRISPR; Excitotoxicity; Humanities and Social Sciences; Motor neurons; multidisciplinary; Mutation; Neurons; Permeability; Pluripotency; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Stem cells; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-017-02729-0

Mutations in C9ORF72 are the most common cause of familial amyotrophic lateral sclerosis (ALS). Here, through a combination of RNA-Seq and electrophysiological studies on induced pluripotent stem cell (iPSC)-derived motor neurons (MNs), we show that increased expression of GluA1 AMPA receptor (AMPAR) subunit occurs in MNs with C9ORF72 mutations that leads to increased Ca 2+ -permeable AMPAR expression and results in enhanced selective MN vulnerability to excitotoxicity. These deficits are not found in iPSC-derived cortical neurons and are abolished by CRISPR/Cas9-mediated correction of the C9ORF72 repeat expansion in MNs. We also demonstrate that MN-specific dysregulation of AMPAR expression is also present in C9ORF72 patient post-mortem material. We therefore present multiple lines of evidence for the specific upregulation of GluA1 subunits in human mutant C9ORF72 MNs that could lead to a potential pathogenic excitotoxic mechanism in ALS. Repeat expansion mutation in C9ORF72 is the most common cause of familial ALS. Here, the authors generate motor neurons from cells of patients with C9ORF72 mutations, and characterize changes in gene expression in these motor neurons compared to genetically corrected lines, which suggest that glutamate receptor subunit GluA1 is dysregulated in this form of ALS.