TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease

• Published in: Scientific reports Vol. 9; no. 1; p. 7229
• Main Authors: Duarte, Victor Hugo Rezende, Miranda, Carolinne Thaisa de Oliveira Fernandes, Cruz, Marina Sampaio, de Araújo, Jéssica Nayara Góes, Duarte, Mychelle Kytchia Rodrigues Nunes, Santos, Ayda Maria Quirino Silva dos, Santos, Isabelle Cristina Clemente dos, Santos, Jéssica Cavalcante dos, da Silva, Ananília Medeiros Gomes, de Oliveira, Juliana Marinho, Paiva, Maria Sanali Moura de Oliveira, de Oliveira Galvão, Marcos Felipe, Rezende, Adriana Augusto, Hirata, Mario Hiroyuki, Hirata, Rosario Dominguez Crespo, Luchessi, André Ducati, Silbiger, Vivian Nogueira
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10.05.2019; Nature Publishing Group
• Subjects: 38/22; 38/39; 38/71; 38/77; 631/337/2019; 631/61/212/2019; 692/4019/592/2193; 692/4019/592/75/2/1674; 692/53/2421; Adult; Aged; Alleles; Arteriosclerosis; Atherosclerosis; Atherosclerosis - complications; Atherosclerosis - pathology; Blood; Cardiovascular disease; Coronary artery; Coronary Artery Disease - complications; Coronary Artery Disease - diagnosis; Coronary Artery Disease - genetics; Coronary vessels; Female; Gene expression; Gene Frequency; Genetic Predisposition to Disease; Genotype; Genotypes; Heart diseases; Humanities and Social Sciences; Humans; Lesions; Leukocytes; Leukocytes - cytology; Leukocytes - metabolism; Male; Middle Aged; multidisciplinary; Myeloid cells; Polymorphism, Single Nucleotide; Receptors, Immunologic - genetics; Receptors, Immunologic - metabolism; RNA, Messenger - metabolism; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-43745-y

Members of the triggering receptor expressed on myeloid cells (TREM) family are associated with atherosclerosis risk and progression. TREML4 is upregulated in the early phase of acute coronary syndrome. We investigated the relationship between the mRNA expression of 13 genes in blood leukocytes, TREML4 polymorphisms, and coronary artery lesion extension (Friesinger index) in patients with coronary artery disease (CAD) (n = 137). TREML4 rs2803495 (A > G) and rs2803496 (T > C) variants and leukocyte mRNA expression were analysed by qRT-PCR. TREML4 expression was higher in patients with major coronary artery lesions than in subjects without or with low and intermediate lesions ( p  < 0.05). However, TREML4 polymorphisms were not associated with coronary lesion extent. Presence of the rs2803495 G allele was not associated with increased TREML4 mRNA expression. Patients carrying the rs2803496 C allele (TC/CC genotypes) were more likely to express TREML4 mRNA than non-C allele carriers (allele C: OR 7.3, and 95% CI 1.9–27.5, p  = 0.03). In conclusion, increased TREML4 mRNA expression in blood leukocytes is influenced by gene polymorphisms and is associated with more severe coronary artery lesions, suggesting its potential as a biomarker of the extent of coronary lesions in patients with CAD.