DNA Ejection from an Archaeal Virus—A Single-Molecule Approach

• Published in: Biophysical journal Vol. 104; no. 10; pp. 2264 - 2272
• Main Authors: Hanhijärvi, K.J., Ziedaite, G., Pietilä, M.K., Hæggström, E., Bamford, D.H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.05.2013; Biophysical Society; The Biophysical Society
• Subjects: Archaeal Viruses - chemistry; Archaeal Viruses - metabolism; Archaeal Viruses - physiology; bacteriophages; Biophysics; capsid; Capsid Proteins - metabolism; Deoxyribonucleic acid; DNA; DNA, Viral - chemistry; DNA, Viral - metabolism; energy; Genetics; genome; Genomics; Haloarcula; Haloarcula - virology; in vitro studies; magnesium; Magnesium - chemistry; Nucleic acids; Osmosis; Osmotic Pressure; polyethylene glycol; Proteins and Nucleic Acids; sodium; Sodium - chemistry; transfer DNA; Translocation; Viral infections; Virus Internalization; Viruses
• ISSN: 0006-3495; 1542-0086; 1542-0086
• DOI: 10.1016/j.bpj.2013.03.061

The translocation of genetic material from the viral capsid to the cell is an essential part of the viral infection process. Whether the energetics of this process is driven by the energy stored within the confined nucleic acid or cellular processes pull the genome into the cell has been the subject of discussion. However, in vitro studies of genome ejection have been limited to a few head-tailed bacteriophages with a double-stranded DNA genome. Here we describe a DNA release system that operates in an archaeal virus. This virus infects an archaeon Haloarcula hispanica that was isolated from a hypersaline environment. The DNA-ejection velocity of His1, determined by single-molecule experiments, is comparable to that of bacterial viruses. We found that the ejection process is modulated by the external osmotic pressure (polyethylene glycol (PEG)) and by increased ion (Mg2+ and Na+) concentration. The observed ejection was unidirectional, randomly paused, and incomplete, which suggests that cellular processes are required to complete the DNA transfer.