A Synthetic Approach Reveals Extensive Tunability of Auxin Signaling

Explaining how the small molecule auxin triggers diverse yet specific responses is a long-standing challenge in plant biology. An essential step in auxin response is the degradation of Auxin/Indole-3-Acetic Acid (Aux/IAA, referred to hereafter as IAA) repressor proteins through interaction with auxi...

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Published inPlant physiology (Bethesda) Vol. 160; no. 1; pp. 135 - 142
Main Authors Havens, Kyle A., Guseman, Jessica M., Jang, Seunghee S., Pierre-Jerome, Edith, Bolten, Nick, Klavins, Eric, Nemhauser, Jennifer L.
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.09.2012
SeriesFocus Issue on Ubiquitin in Plant Biology
Subjects
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Auxins
F box proteins
F-Box Proteins - genetics
F-Box Proteins - metabolism
Flow Cytometry
Fluorescence
Half lives
Half-Life
indole acetic acid
Indoleacetic Acids - metabolism
Indoleacetic Acids - pharmacology
Insulin antibodies
mathematical models
Models, Biological
Parametric models
Plant Growth Regulators - metabolism
Plants, Genetically Modified - drug effects
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Protein Structure, Tertiary
Proteolysis
Receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
repressor proteins
Repressor Proteins - metabolism
s - Focus Issue
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Seedlings
Signal Transduction
Species Specificity
Time Factors
Transformation, Genetic
Truncation
Ubiquitination
Yeasts
Online AccessGet full text
ISSN0032-0889
1532-2548
1532-2548
DOI10.1104/pp.112.202184

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Summary:Explaining how the small molecule auxin triggers diverse yet specific responses is a long-standing challenge in plant biology. An essential step in auxin response is the degradation of Auxin/Indole-3-Acetic Acid (Aux/IAA, referred to hereafter as IAA) repressor proteins through interaction with auxin receptors. To systematically characterize diversity in degradation behaviors among IAA ǀ receptor pairs, we engineered auxin-induced degradation of plant IAA proteins in yeast (Saccharomyces cerevisiae). We found that IAA degradation dynamics vary widely, depending on which receptor is present, and are not encoded solely by the degron-containing domain II. To facilitate this and future studies, we identified a mathematical model able to quantitatively describe IAA degradation behavior in a single parameter. Together, our results demonstrate the remarkable tunability conferred by specific configurations of the auxin response pathway.
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Some figures in this article are displayed in color online but in black and white in the print edition.
www.plantphysiol.org/cgi/doi/10.1104/pp.112.202184
These authors contributed equally to the article.
This work was supported by the Paul G. Allen Family Foundation and the National Science Foundation (grant nos. CISE–0832773 to E.K. and IOS–0919021 to J.L.N.). J.M.G. was supported by the National Institutes of Health (training grant no. T32HD007183). E.P.J. was supported by a National Science Foundation Graduate Research Fellowship and the Seattle Chapter of the Achievement Rewards for College Scientists Foundation.
The online version of this article contains Web-only data.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jennifer L. Nemhauser (jn7@uw.edu).
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ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.112.202184