The emergence of the brain non-CpG methylation system in vertebrates

• Published in: Nature ecology & evolution Vol. 5; no. 3; pp. 369 - 378
• Main Authors: de Mendoza, Alex, Poppe, Daniel, Buckberry, Sam, Pflueger, Jahnvi, Albertin, Caroline B., Daish, Tasman, Bertrand, Stephanie, de la Calle-Mustienes, Elisa, Gómez-Skarmeta, José Luis, Nery, Joseph R., Ecker, Joseph R., Baer, Boris, Ragsdale, Clifton W., Grützner, Frank, Escriva, Hector, Venkatesh, Byrappa, Bogdanovic, Ozren, Lister, Ryan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2021; Nature Publishing Group; Nature
• Subjects: 38/39; 45/22; 45/23; 631/181/2806; 631/181/735; 631/208/177; 631/208/212/748; 64/114; 64/116; 64/60; Animals; Biochemistry, Molecular Biology; Biological and Physical Anthropology; Biomedical and Life Sciences; Brain; Brain - metabolism; Canonical forms; Cognition; Cognitive ability; CpG islands; Deoxyribonucleic acid; DNA; DNA Methylation; Ecology; Epigenetics; Evolutionary Biology; Genome; Genomes; Genomics; Life Sciences; Mammals; MeCP2 protein; Methyl-CpG binding protein; Methyl-CpG-Binding Protein 2 - genetics; Methyl-CpG-Binding Protein 2 - metabolism; Paleontology; Reproduction (copying); Rett syndrome; Transcription; Vertebrates; Vertebrates - genetics; Zoology; amphioxus; Evolution; methylation
• ISSN: 2397-334X; 2397-334X
• DOI: 10.1038/s41559-020-01371-2

Mammalian brains feature exceptionally high levels of non-CpG DNA methylation alongside the canonical form of CpG methylation. Non-CpG methylation plays a critical regulatory role in cognitive function, which is mediated by the binding of MeCP2, the transcriptional regulator that when mutated causes Rett syndrome. However, it is unclear whether the non-CpG neural methylation system is restricted to mammalian species with complex cognitive abilities or has deeper evolutionary origins. To test this, we investigated brain DNA methylation across 12 distantly related animal lineages, revealing that non-CpG methylation is restricted to vertebrates. We discovered that in vertebrates, non-CpG methylation is enriched within a highly conserved set of developmental genes transcriptionally repressed in adult brains, indicating that it demarcates a deeply conserved regulatory program. We also found that the writer of non-CpG methylation, DNMT3A, and the reader, MeCP2, originated at the onset of vertebrates as a result of the ancestral vertebrate whole-genome duplication. Together, we demonstrate how this novel layer of epigenetic information assembled at the root of vertebrates and gained new regulatory roles independent of the ancestral form of the canonical CpG methylation. This suggests that the emergence of non-CpG methylation may have fostered the evolution of sophisticated cognitive abilities found in the vertebrate lineage. By studying brain DNA methylation across 13 distantly related animals, the authors show that non-CpG DNA methylation, which plays a regulatory role in cognition, is restricted to vertebrates and was assembled at the origin of the vertebrate lineage as a result of the ancestral vertebrate whole-genome duplication.