Plant Hormonomics Multiple Phytohormone Profiling by Targeted Metabolomics

Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant materia...

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Published inPlant physiology (Bethesda) Vol. 177; no. 2; pp. 476 - 489
Main Authors Šimura, Jan, Antoniadi, Ioanna, Široká, Jitka, Tarkowská, Danuše, Strnad, Miroslav, Ljung, Karin, Novák, Ondřej
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.06.2018
Subjects
Botanik
Botany
Breakthrough Technologies
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ISSN0032-0889
1532-2548
1532-2548
DOI10.1104/pp.18.00293

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Summary:Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
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www.plantphysiol.org/cgi/doi/10.1104/pp.18.00293
Ja.Š., I.A., K.L., and O.N. designed the study; Ja.Š., D.T., and O.N. participated in development of the experimental protocol; Ja.Š. performed most of the experiments; Ja.Š., I.A., and Ji.Š. analyzed data; Ja.Š. and O.N. wrote the article with contributions of all the authors; D.T., M.S., K.L., and O.N. supervised the research.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Ondřej Novák (novako@ueb.cas.cz).
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.18.00293