Validity of the twitch interpolation technique for the assessment of quadriceps neuromuscular asymmetries

• Published in: Journal of electromyography and kinesiology Vol. 28; pp. 31 - 36
• Main Authors: Maffiuletti, Nicola Angelo, Barbero, Marco, Cescon, Corrado, Clijsen, Ron, Beretta-Piccoli, Matteo, Schneebeli, Alessandro, Preiss, Stefan, Togninalli, Danilo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2016
• Subjects: Activation; Adult; Anterior Cruciate Ligament Injuries - diagnosis; Anterior Cruciate Ligament Injuries - physiopathology; Anterior Cruciate Ligament Injuries - surgery; Anterior Cruciate Ligament Reconstruction - adverse effects; Anterior Cruciate Ligament Reconstruction - trends; Contractility; Cross-Sectional Studies; Female; Follow-Up Studies; Humans; Male; Muscle Contraction - physiology; Muscle, Skeletal - physiology; Physical Medicine and Rehabilitation; Quadriceps Muscle - physiology; Reproducibility of Results; Strength; Transcutaneous Electric Nerve Stimulation - methods; Transcutaneous Electric Nerve Stimulation - standards; Weakness; Young Adult; Activation; Weakness; Strength; Contractility
• ISSN: 1050-6411; 1873-5711; 1873-5711
• DOI: 10.1016/j.jelekin.2016.03.002

This study examined the validity of the twitch interpolation technique for evaluating side-to-side asymmetries in quadriceps neuromuscular function. Fifty-six subjects with a wide range of asymmetries (19 healthy, 24 with unilateral and 13 with bilateral anterior cruciate ligament reconstruction) took part in the study. Supramaximal electrical paired stimuli were delivered to the quadriceps muscle during and immediately after a maximal voluntary contraction (MVC) of the knee extensors (twitch interpolation technique). MVC torque, voluntary activation and resting doublet-evoked torque were measured separately for the two sides, and percent side-to-side asymmetries were calculated for each parameter. MVC torque asymmetry was plotted against voluntary activation asymmetry and doublet-evoked torque asymmetry, and a multiple regression analysis was also conducted. Significant positive correlations were observed between MVC torque asymmetry and both voluntary activation asymmetry (r=0.40; p=0.002) and doublet-evoked torque asymmetry (r=0.53; p<0.001), and their relative contribution to MVC torque asymmetry was comparable (r=0.64; p<0.001). These results establish the validity of the twitch interpolation technique for the assessment of neuromuscular asymmetries. This methodology could provide useful insights into the contribution of some neural and muscular mechanisms that underlie quadriceps strength deficits.