Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women

• Published in: Biomolecules (Basel, Switzerland) Vol. 15; no. 5; p. 739
• Main Authors: Díaz-Del Cerro, Estefanía, Félix, Judith, Martínez-Poyato, Mª Carmen, De la Fuente, Mónica
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.05.2025; MDPI
• Subjects: Age; Aged; Aging; Aging - drug effects; Amino acids; Amino Acids - administration & dosage; Amino Acids - pharmacology; Antioxidants; Antioxidants - administration & dosage; Antioxidants - pharmacology; Antitumor activity; Bioactive compounds; biological age; Blood cells; Carbohydrates; Chemotaxis; Comparative analysis; Cortisol; Dehydroepiandrosterone; Dehydroepiandrosterone - blood; Diabetes; Diet; dietary supplement; Dietary Supplements; Enzymes; Exercise; Female; Fruits; Glutathione - blood; Glutathione - metabolism; Glutathione peroxidase; Health aspects; Homeostasis; Humans; immune functions; Immune response; Immune system; Immunity (Disease); Immunosenescence; Leukocytes (neutrophilic); Lifestyles; Lymphocytes; Measurement; Menopause; Metabolism; Middle Aged; Mitogens; Morbidity; Natural killer cells; Nutrition research; Oxidants; oxidative state; Oxidative stress; Oxidative Stress - drug effects; Peripheral blood; Physiological aspects; Post-menopause; Postmenopausal women; Postmenopause - blood; Postmenopause - drug effects; Processed foods; Sleep; stress hormones; Testing; Vegetables; Vitamins; Vitamins - administration & dosage; Vitamins - pharmacology; Womens health; Spain; oxidative state; stress hormones; immune functions; biological age; postmenopausal women; dietary supplement
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom15050739

Aging involves immune system deterioration (immunosenescence) and increased oxidative stress, both associated with morbidity and mortality. Menopause accelerates aging, highlighting the need for strategies to mitigate its effects in postmenopausal women. This study assessed the impact of daily oral supplementation for one month with 39 bioactive compounds (UNAMINA)—including amino acids, vitamins, and antioxidants—on immune function, redox parameters, stress-related hormones, and biological age in healthy postmenopausal women. Peripheral blood samples were collected before and after supplementation to analyze lymphocyte and neutrophil functions (adherence, chemotaxis, natural killer cell antitumor capacity, and lymphoproliferative response to mitogens), oxidative stress markers (antioxidant defenses such as glutathione peroxidase (GPx) and reductase activities, reduced glutathione (GSH) concentrations, as well as oxidants such as oxidized glutathione (GSSG), and lipid peroxidative damage) in blood cells, and stress-related hormones (dehydroepiandrosterone (DHEA) and cortisol) in plasma. Supplementation improved all immune cell functions and decreased oxidative stress (increasing antioxidants defenses such as GPx activity and GSH concentration and decreasing GSSG amount) and cortisol concentrations, whereas those of DHEA increased. The biological age also decreased. The results suggest that these bioactive compounds may be a beneficial strategy for promoting healthier aging in postmenopausal women by enhancing immune function, reducing biological age, improving redox balance, and regulating stress hormones.