Modulation of sympathetic vasoconstriction is critical for the effects of sleep on arterial pressure in mice

• Published in: The Journal of physiology Vol. 596; no. 4; pp. 591 - 608
• Main Authors: Lo Martire, Viviana, Silvani, Alessandro, Alvente, Sara, Bastianini, Stefano, Berteotti, Chiara, Valli, Alice, Zoccoli, Giovanna
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 15.02.2018; John Wiley and Sons Inc
• Subjects: Acetylcholine receptors (muscarinic); Adrenergic receptors; Animal models; Animals; Anti-Arrhythmia Agents - pharmacology; Arterial Pressure; Atenolol; Atropine; Autonomic nervous system; Baroreceptors; Blood pressure; Blood vessels; Cardiovascular; cardiovascular control; Cardiovascular diseases; Cardiovascular system; Cardiovascular System - drug effects; Cardiovascular System - physiopathology; Circulatory system; EEG; Electromyography; Heart diseases; Heart Rate; Humans; Male; Mice; Mice, Inbred C57BL; NREM sleep; Parasympathetic nervous system; Prazosin; Reflexes; REM sleep; Research Paper; Sleep; Sleep and wakefulness; Sympathetic Nervous System; Vasoconstriction; Wakefulness; sleep; cardiovascular control; mice
• ISSN: 0022-3751; 1469-7793; 1469-7793
• DOI: 10.1113/JP275353

Key points While values of arterial pressure during sleep are predictive of cardiovascular risk, the autonomic mechanisms underlying the cardiovascular effects of sleep remain poorly understood. Here, we assess the autonomic mechanisms of the cardiovascular effects of sleep in C57Bl/6J mice, taking advantage of a novel technique for continuous intraperitoneal infusion of autonomic blockers. Our results indicate that non‐REM sleep decreases arterial pressure by decreasing sympathetic vasoconstriction, decreases heart rate by balancing parasympathetic activation and sympathetic withdrawal, and increases cardiac baroreflex sensitivity mainly by increasing fluctuations in parasympathetic activity. Our results also indicate that REM sleep increases arterial pressure by increasing sympathetic activity to the heart and blood vessels, and increases heart rate, at least in part, by increasing cardiac sympathetic activity. These results provide a framework for generating and testing hypotheses on cardiovascular derangements during sleep in mouse models and human patients. The values of arterial pressure (AP) during sleep predict cardiovascular risk. Sleep exerts similar effects on cardiovascular control in human subjects and mice. We aimed to determine the underlying autonomic mechanisms in 12 C57Bl/6J mice with a novel technique of intraperitoneal infusion of autonomic blockers, while monitoring the electroencephalogram, electromyogram, AP and heart period (HP, i.e. 1/heart rate). In different sessions, we administered atropine methyl nitrate, atenolol and prazosin to block muscarinic cholinergic, β1‐adrenergic and α1‐adrenergic receptors, respectively, and compared each drug infusion with a matched vehicle infusion. The decrease in AP from wakefulness to non‐rapid‐eye‐movement sleep (N) was abolished by prazosin but was not significantly affected by atropine and atenolol, which, however, blunted the accompanying increase in HP to a similar extent. On passing from N to rapid‐eye‐movement sleep (R), the increase in AP was significantly blunted by prazosin and atenolol, whereas the accompanying decrease in HP was blunted by atropine and abolished by atenolol. Cardiac baroreflex sensitivity (cBRS, sequence technique) was dramatically decreased by atropine and slightly increased by prazosin. These data indicate that in C57Bl/6J mice, N decreases mean AP by decreasing sympathetic vasoconstriction, increases HP by balancing parasympathetic activation and sympathetic withdrawal, and increases cBRS mainly by increasing fluctuations in parasympathetic activity. R increases mean AP by increasing sympathetic vasoconstriction and cardiac sympathetic activity, which also explains, at least in part, the concomitant decrease in HP. These data represent the first comprehensive assessment of the autonomic mechanisms of cardiovascular control during sleep in mice. Key points While values of arterial pressure during sleep are predictive of cardiovascular risk, the autonomic mechanisms underlying the cardiovascular effects of sleep remain poorly understood. Here, we assess the autonomic mechanisms of the cardiovascular effects of sleep in C57Bl/6J mice, taking advantage of a novel technique for continuous intraperitoneal infusion of autonomic blockers. Our results indicate that non‐REM sleep decreases arterial pressure by decreasing sympathetic vasoconstriction, decreases heart rate by balancing parasympathetic activation and sympathetic withdrawal, and increases cardiac baroreflex sensitivity mainly by increasing fluctuations in parasympathetic activity. Our results also indicate that REM sleep increases arterial pressure by increasing sympathetic activity to the heart and blood vessels, and increases heart rate, at least in part, by increasing cardiac sympathetic activity. These results provide a framework for generating and testing hypotheses on cardiovascular derangements during sleep in mouse models and human patients.