Detection of cytomegalovirus infection during clinical trials of glycoprotein B vaccine
Preabsorption of sera with cytomegalovirus (CMV ) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of s...
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| Published in | Vaccine Vol. 23; no. 4; pp. 507 - 510 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Elsevier Ltd
09.12.2004
Elsevier Elsevier Limited |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0264-410X 1873-2518 |
| DOI | 10.1016/j.vaccine.2004.06.027 |
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| Abstract | Preabsorption of sera with cytomegalovirus
(CMV
) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection. |
|---|---|
| AbstractList | Preabsorption of sera with cytomegalovirus (CMV) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection.Preabsorption of sera with cytomegalovirus (CMV) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection. Preabsorption of sera with cytomegalovirus(CMV)glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection. Preabsorption of sera with cytomegalovirus (CMV ) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection. |
| Author | Zhang, Changpin Pass, Robert F. |
| Author_xml | – sequence: 1 givenname: Changpin surname: Zhang fullname: Zhang, Changpin organization: Department of Pediatrics, UAB School of Medicine, Children’s Hospital CHB 309, 1600 7th Avenue South, Birmingham, AL 35233, USA – sequence: 2 givenname: Robert F. surname: Pass fullname: Pass, Robert F. email: rpass@peds.uab.edu organization: Department of Pediatrics, UAB School of Medicine, Children’s Hospital CHB 309, 1600 7th Avenue South, Birmingham, AL 35233, USA |
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| References | Zanghellini, Boppana, Emery, Griffiths, Pass (bib6) 1999; 180 Spaete, Thayer, Probert, Masiarz, Chamberlain, Rasmussen (bib7) 1988; 167 Frey, Harrison, Pass, Yang, Boken, Sekulovich (bib5) 1999; 180 Pass, Duliege, Boppana, Sekulovich, Percell, Britt (bib4) 1999; 180 Stagno, Pass, Dworsky, Alford (bib2) 1982; 25 Griffiths, Baboonian (bib3) 1984; 91 Plotkin, Starr, Friedman, Brayman, Harris, Jackson (bib1) 1991; 114 |
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(CMV
) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during... Preabsorption of sera with cytomegalovirus (CMV) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during... Preabsorption of sera with cytomegalovirus(CMV)glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during... |
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| SubjectTerms | Absorption Alabama Antibodies, Viral - blood Antigens Applied microbiology Automation Biological and medical sciences Clinical trials CMV infection CMV vaccine Cytomegalovirus Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - immunology Cytomegalovirus Infections - prevention & control Cytomegalovirus Infections - virology Cytomegalovirus Vaccines - administration & dosage Cytomegalovirus Vaccines - immunology Fundamental and applied biological sciences. Psychology Glycoprotein B Human cytomegalovirus Humans Immunoassay Immunoglobulin G - blood Infections Laboratories Microbiology Outcome and Process Assessment (Health Care) Pharmaceutical industry Proteins Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Subunit - administration & dosage Vaccines, Subunit - immunology Viral Envelope Proteins - immunology |
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| Title | Detection of cytomegalovirus infection during clinical trials of glycoprotein B vaccine |
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