Detection of cytomegalovirus infection during clinical trials of glycoprotein B vaccine

• Published in: Vaccine Vol. 23; no. 4; pp. 507 - 510
• Main Authors: Zhang, Changpin, Pass, Robert F.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 09.12.2004; Elsevier; Elsevier Limited
• Subjects: Absorption; Alabama; Antibodies, Viral - blood; Antigens; Applied microbiology; Automation; Biological and medical sciences; Clinical trials; CMV infection; CMV vaccine; Cytomegalovirus; Cytomegalovirus Infections - diagnosis; Cytomegalovirus Infections - immunology; Cytomegalovirus Infections - prevention & control; Cytomegalovirus Infections - virology; Cytomegalovirus Vaccines - administration & dosage; Cytomegalovirus Vaccines - immunology; Fundamental and applied biological sciences. Psychology; Glycoprotein B; Human cytomegalovirus; Humans; Immunoassay; Immunoglobulin G - blood; Infections; Laboratories; Microbiology; Outcome and Process Assessment (Health Care); Pharmaceutical industry; Proteins; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Subunit - administration & dosage; Vaccines, Subunit - immunology; Viral Envelope Proteins - immunology; Alabama; Glycoprotein B; CMV vaccine; CMV infection; Infection; Viral disease; Glycoprotein; Clinical trial; Vaccine; Detection
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.06.027

Preabsorption of sera with cytomegalovirus (CMV ) glycoprotein B (gB) prior to testing for CMV IgG antibody was evaluated for detection of CMV infection during CMV gB vaccine clinical trials. Although, 98.2% of 109 CMV gB vaccine recipients were seropositive with a standard assay, preabsorption of sera with gB reduced their CMV antibody to extremely low levels compared with subjects with CMV infection. One subject who acquired CMV during a vaccine trial was easily identified by gB-absorbed CMV IgG results. The gB-absorbed CMV IgG antibody assay is a promising approach for screening CMV gB vaccine trial participants for CMV infection.