Alkali solution extraction of rice residue protein isolates: Influence of alkali concentration on protein functional, structural properties and lysinoalanine formation

• Published in: Food chemistry Vol. 218; pp. 207 - 215
• Main Authors: Hou, Furong, Ding, Wenhui, Qu, Wenjuan, Oladejo, Ayobami Olayemi, Xiong, Feng, Zhang, Weiwei, He, Ronghai, Ma, Haile
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2017
• Subjects: Alkalies - chemistry; Alkalies - pharmacology; Alkaline extraction; crude protein; disulfide bonds; emulsifying; foaming properties; Functional and structural properties; high performance liquid chromatography; Hydrophobic and Hydrophilic Interactions; hydrophobicity; Lysinoalanine; Lysinoalanine - analysis; Lysinoalanine - metabolism; Oryza - metabolism; Plant Extracts - chemistry; Plant Extracts - metabolism; Plant Proteins - metabolism; protein isolates; rice; RRPI; Solubility; thiols; Lysinoalanine; Alkaline extraction; Functional and structural properties; RRPI
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2016.09.064

•Alkaline concentration above 0.03M may cause nutrient or safety problems in extraction of rice residue protein.•Alkaline extraction may influence functional or structural properties of rice residue protein.•The LAL formation depends on the alkali concentration. This study evaluated the nutrient property and safety of the rice residue protein isolates (RRPI) product (extracted by different alkali concentrations) by exploring the protein functional, structural properties and lysinoalanine (LAL) formation. The results showed that with the rising of alkali concentration from 0.03M to 0.15M, the solubility, emulsifying and foaming properties of RRPI increased at first and then descended. When the alkali concentration was greater than 0.03M, the RRPI surface hydrophobicity decreased and the content of thiol and disulfide bond, Lys and Cys significantly reduced. By the analysis of HPLC, the content of LAL rose up from 276.08 to 15,198.07mg/kg and decreased to 1340.98mg/kg crude protein when the alkali concentration increased from 0.03 to 0.09M and until to 0.15M. These results indicated that RRPI alkaline extraction concentration above 0.03M may cause severe nutrient or safety problems of protein.