Evaluation of the Effects of Autologous Leukocyte- and Platelet-Rich Fibrin Membranes for Treating Chronic Wounds: A Prospective Study

• Published in: Animals (Basel) Vol. 15; no. 1; p. 112
• Main Authors: Aragosa, Federica, Fatone, Gerardo, Caterino, Chiara, Cavalli, Stefano, Piscitelli, Alfonso, Vallefuoco, Rosario, Lamagna, Francesco, Della Valle, Giovanni
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2025; MDPI
• Subjects: Animals; Antibiotics; Blood platelets; Dogs; Edema; Fibrin; Health aspects; Infections; L-PRF; Leukocytes; Membranes; Metronidazole; platelet concentrates; regenerative medicine; second intention; spontaneous wounds; Vascular endothelial growth factor; Veterinary medicine; Wound healing; Wounds and injuries; United States; United States > US; second intention; platelet concentrates; spontaneous wounds; regenerative medicine; L-PRF
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani15010112

(1) Background: The aim of this prospective study was to evaluate the efficacy of topical application of leukocyte- and platelet-rich fibrin (L-PRF) membranes for treating chronic cutaneous wounds in dogs. (2) Methods: Chronic wounds unresponsive to conventional treatments were assessed on digital photographs and classified using the Bates-Jensen Wound Assessment Tool (BWAT). Each lesion was treated with L-PRF membranes. Epithelialization, granulation tissue formation, wound healing rate, and contraction were statistically analyzed during follow-up. (3) Results: All but one of the cases were treated with a single application of L-PRF for a median of 30 days after onset. All wounds healed after a median of 22 days. The BWAT score, total wound area, and granulation tissue decreased significantly over time while wound contraction increased. The most significant reduction in the total wound area was observed within the first 3 weeks after treatment. None of the dogs required the administration of antibiotics. (4) Conclusions: L-PRF membrane promoted the wound healing process in dogs and is a valuable and cost-effective tool for treating chronic wounds, thereby reducing the need for antibiotics administration.