Cloning and characterisation of the human adenosine A3 receptor gene

• Published in: FEBS letters Vol. 384; no. 3; pp. 243 - 246
• Main Authors: Murrison, E.M., Goodson, S.J., Edbrooke, M.R., Harris, C.A.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 22.04.1996
• Subjects: Adenosine A3 receptor; Amino Acid Sequence; Base Sequence; Blotting, Northern; DNA, Complementary - chemistry; Humans; Intron; Introns; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction - methods; Receptors, Purinergic P1 - chemistry; Receptors, Purinergic P1 - genetics; Receptors, Purinergic P1 - isolation & purification; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; TATA Box; Tissue Distribution; Transcription, Genetic; Upstream sequence; Upstream sequence; Intron; Adenosine A3 receptor
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(96)00324-9

We have cloned the gene for the human adenosine A3 receptor and report characterisation of its intron/exon structure and upstream untranslated region. The open reading frame is interrupted by a single intron of approximately 2.2 kb, within the coding sequence for the second cytoplasmic loop. Sequence analysis of the upstream region reveals no TATA box but the transcriptional start site has been mapped to a common nucleotide in three tissues by 5′-RACE and RT-PCR analysis. Northern blotting, 5′-RACE PCR and analysis of upstream sequences, have provided no evidence for the occurrence of further introns in the upstream untranslated sequence or of transcriptional regulation by alternative splicing in this region.