A Method for Individualizing the Prediction of Immunogenicity of Protein Vaccines and Biologic Therapeutics : Individualized T Cell Epitope Measure (iTEM)

• Published in: BioMed research international Vol. 2010; no. 2010; pp. 1 - 7
• Main Authors: Cohen, Tobias, Moise, Leonard, Ardito, Matthew, Martin, William, De Groot, Anne S.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2010; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Alleles; Amino Acid Sequence; Animals; Antigens - chemistry; Antigens - immunology; Chi-Square Distribution; Computational Biology - methods; Epitopes, T-Lymphocyte - immunology; Haplotypes; HLA Antigens - immunology; HLA-A2 Antigen - immunology; HLA-DR Antigens - immunology; HLA-DR1 Antigen - immunology; HLA-DRB1 Chains; Human subjects; Immune system; Immunology; Linear Models; Lymphocyte Activation; Methodology Report; Mice; Mice, Transgenic; Peptides; Proteins - chemistry; Proteins - immunology; Reproducibility of Results; Software; Studies; T cell receptors; T-Lymphocytes - immunology; Vaccines, Subunit - chemistry; Vaccines, Subunit - immunology
• ISSN: 1110-7243; 2314-6133; 1110-7251; 1110-7251; 2314-6141
• DOI: 10.1155/2010/961752

The promise of pharmacogenomics depends on advancing predictive medicine. To address this need in the area of immunology, we developed the individualized T cell epitope measure (iTEM) tool to estimate an individual's T cell response to a protein antigen based on HLA binding predictions. In this study, we validated prospective iTEM predictions using data from in vitro and in vivo studies. We used a mathematical formula that converts DRB1∗ allele binding predictions generated by EpiMatrix, an epitope-mapping tool, into an allele-specific scoring system. We then demonstrated that iTEM can be used to define an HLA binding threshold above which immune response is likely and below which immune response is likely to be absent. iTEM's predictive power was strongest when the immune response is focused, such as in subunit vaccination and administration of protein therapeutics. iTEM may be a useful tool for clinical trial design and preclinical evaluation of vaccines and protein therapeutics.