Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid

• Published in: Biotechnology letters Vol. 37; no. 1; pp. 205 - 209
• Main Authors: Mhlongo, M. I, Piater, L. A, Steenkamp, P. A, Madala, N. E, Dubery, I. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.01.2015; Springer Netherlands; Springer Nature B.V
• Subjects: acibenzolar-S-methyl; Applied Microbiology; Biochemistry; Biological; Biomedical and Life Sciences; Biotechnology; chitosan; chlorogenic acid; Chlorogenic Acid - chemistry; Chlorogenic Acid - metabolism; cis-trans isomers; Exposure; Fingerprinting; flagellin; Gene Expression Regulation, Plant - genetics; leaves; Life Sciences; lipopolysaccharides; Metabolites; Metabolome - genetics; metabolomics; Metabolomics - methods; Microbiology; Nicotiana - genetics; Nicotiana - metabolism; Original Research Paper; Origins; Plant biology; Plant tissues; Plants (organisms); Pools; Tandem Mass Spectrometry; Tobacco; Ultraviolet radiation; 5-caffeoylquinic acid; Natural products; Chlorogenic acids; Tobacco cells; Plants; Phytoanticipins; Secondary metabolism
• ISSN: 0141-5492; 1573-6776; 1573-6776
• DOI: 10.1007/s10529-014-1645-8

Previous studies suggest that only trans-isomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to sunlight. Very little, however, is known about the biological significance and origin of cis-isomers. Here we show for the first time the accumulation of cis-5-caffeoylquinic acid in cultured tobacco cells treated with different inducers of plant defence (lipopolysaccharides, flagellin peptide-22, chitosan, acibenzolar-S-methyl and isonitrosoacetophenone), without exposure to UV light and with a 2-fold (on average) increase in the concentration of the pool in comparison to non-stimulated cells. Our UHPLC–Q-TOF-MS and multivariate statistical results suggest the presence of a possible biological pathway responsible for the production of cis-CGAs in tobacco plants.