Study of polyamines during grape ripening indicate an important role of polyamine catabolism

• Published in: Plant physiology and biochemistry Vol. 67; pp. 105 - 119
• Main Authors: Agudelo-Romero, Patricia, Bortolloti, Cristina, Pais, Maria Salomé, Tiburcio, Antonio Fernández, Fortes, Ana Margarida
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.06.2013; Elsevier
• Subjects: Amine Oxidase (Copper-Containing) - metabolism; arginine decarboxylase; Biological and medical sciences; Copper amine oxidase; cultivars; developmental stages; diamine oxidase; enzyme activity; fruiting; fruits; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant; genes; Grape ripening; grapes; growth regulators; hydrogen peroxide; metabolism; Oxidoreductases Acting on CH-NH Group Donors - metabolism; Plant physiology and development; Polyamine catabolism; Polyamine Oxidase; Polyamines - metabolism; putrescine; Putrescine - metabolism; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; ripening; small fruits; spermidine; spermine; Spermine - metabolism; Vitis; Vitis - enzymology; Vitis - genetics; Vitis - metabolism; Vitis vinifera; ADC; CuAO; Grape ripening; PAs; Polyamine oxidase; SPMS; Copper amine oxidase; Polyamine catabolism; SPDS; Gene expression; Vitis vinifera; SAMDC; S; SH; PH; SAM; PAO; Enzyme; Oxidase; Ripening; Amine; Plant physiology; Fruit crop; Vitidaceae; Dicotyledones; Grape; Angiospermae; Spermatophyta; Catabolism; Oxidoreductases; Copper
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2013.02.024

Grapevine (Vitis species) is the most economically important fruit crop worldwide. Ripening of non-climacteric fruits such as grapes has been the subject of intense research. Despite this interest, little is known on the role played by polyamines in the onset of ripening of non-climacteric fruits. These growth regulators have been involved in plant development and stress responses. Molecular and biochemical studies were developed in three important Portuguese cultivars (Trincadeira, Touriga Nacional and Aragonês) during the year 2008 and in Trincadeira during 2007 in order to gather insights on the role of polyamines in grape ripening. Microarray and real-time qPCR studies revealed up-regulation of a gene coding for arginine decarboxylase (ADC) during grape ripening in all the varieties. This increase was not accompanied by an increase in free and conjugated polyamines that presented a strong decrease. Putrescine and Spermidine levels were higher at earlier stages of development, while Spermine level remained constant. Berries of Trincadeira cultivar presented the highest content in total free and conjugated polyamines at earlier stages of fruit development in particular in the year 2007. The decrease in polyamines content during grape ripening was accompanied by up-regulation of genes coding for diamine oxidase (CuAO) and polyamine oxidase (PAO), together with a significant increase in their enzymatic activity and in the hydrogen peroxide content. These results provide, for the first time, strong evidence of a role of polyamine catabolism in grape ripening possibly through interaction with other growth regulators. ► Study of polyamine metabolism during grape ripening. ► The expression of genes involved in polyamine synthesis and catabolism was analyzed. ► It was examined the polyamine content and the activity of enzymes involved in polyamine catabolism. ► There is increase in polyamine catabolism and decrease in polyamine content during grape ripening.