Identification of Novel Diagnostic and Prognostic Gene Signature Biomarkers for Breast Cancer Using Artificial Intelligence and Machine Learning Assisted Transcriptomics Analysis

• Published in: Cancers Vol. 15; no. 12; p. 3237
• Main Authors: Mirza, Zeenat, Ansari, Md Shahid, Iqbal, Md Shahid, Ahmad, Nesar, Alganmi, Nofe, Banjar, Haneen, Al-Qahtani, Mohammed H., Karim, Sajjad
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.06.2023; MDPI
• Subjects: Accuracy; Analysis; Artificial intelligence; Biological markers; Biomarkers; Breast cancer; Cancer; Classification; CXCL10 protein; Data analysis; Datasets; Diagnosis; DNA microarrays; Feature selection; Gene expression; Genes; Genetic aspects; Human error; Learning algorithms; Leukemia; Machine learning; Medical prognosis; Metaphase; Ontology; Prognosis; PTEN protein; Sample size; Senescence; Statistical analysis; Survival; Survival analysis; Transcriptomics; Tumors; Saudi Arabia; Canada; United States > US; machine learning methods; diagnostic and prognostic model; breast cancer; gene expression profiling; artificial intelligence
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers15123237

Background: Breast cancer (BC) is one of the most common female cancers. Clinical and histopathological information is collectively used for diagnosis, but is often not precise. We applied machine learning (ML) methods to identify the valuable gene signature model based on differentially expressed genes (DEGs) for BC diagnosis and prognosis. Methods: A cohort of 701 samples from 11 GEO BC microarray datasets was used for the identification of significant DEGs. Seven ML methods, including RFECV-LR, RFECV-SVM, LR-L1, SVC-L1, RF, and Extra-Trees were applied for gene reduction and the construction of a diagnostic model for cancer classification. Kaplan–Meier survival analysis was performed for prognostic signature construction. The potential biomarkers were confirmed via qRT-PCR and validated by another set of ML methods including GBDT, XGBoost, AdaBoost, KNN, and MLP. Results: We identified 355 DEGs and predicted BC-associated pathways, including kinetochore metaphase signaling, PTEN, senescence, and phagosome-formation pathways. A hub of 28 DEGs and a novel diagnostic nine-gene signature (COL10A, S100P, ADAMTS5, WISP1, COMP, CXCL10, LYVE1, COL11A1, and INHBA) were identified using stringent filter conditions. Similarly, a novel prognostic model consisting of eight-gene signatures (CCNE2, NUSAP1, TPX2, S100P, ITM2A, LIFR, TNXA, and ZBTB16) was also identified using disease-free survival and overall survival analysis. Gene signatures were validated by another set of ML methods. Finally, qRT-PCR results confirmed the expression of the identified gene signatures in BC. Conclusion: The ML approach helped construct novel diagnostic and prognostic models based on the expression profiling of BC. The identified nine-gene signature and eight-gene signatures showed excellent potential in BC diagnosis and prognosis, respectively.