Salivary MMP-8, TIMP-1, and ICTP as markers of advanced periodontitis

• Published in: Journal of clinical periodontology Vol. 37; no. 6; pp. 487 - 493
• Main Authors: Gursoy, Ulvi Kahraman, Könönen, Eija, Pradhan-Palikhe, Pratikshya, Tervahartiala, Taina, Pussinen, Pirkko J., Suominen-Taipale, Liisa, Sorsa, Timo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2010; Blackwell
• Subjects: Adult; Area Under Curve; Biological and medical sciences; Biomarkers - analysis; Collagen Type I; Enzyme-Linked Immunosorbent Assay; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Fluoroimmunoassay; Gingival Hemorrhage - classification; Humans; ICTP; Male; Matrix Metalloproteinase 14 - analysis; Matrix Metalloproteinase 8 - analysis; Medical sciences; Middle Aged; MMP-14; MMP-8; Non tumoral diseases; Otorhinolaryngology. Stomatology; Peptide Fragments - analysis; Peptides; Periodontal Index; Periodontal Pocket - classification; Periodontitis - diagnosis; Procollagen - analysis; ROC Curve; saliva; Saliva - chemistry; Saliva - enzymology; Smoking; TIMP-1; Tissue Inhibitor of Metalloproteinase-1 - analysis; Periodontal disease; TIMP-1; Periodontitis; ICTP; Stomatology; MMP-14; MMP-8; Dentistry; Saliva
• ISSN: 0303-6979; 1600-051X; 1600-051X
• DOI: 10.1111/j.1600-051X.2010.01563.x

Gursoy UK, Könönen E, Pradhan‐Palikhe P, Tervahartiala T, Pussinen PJ, Suominen‐Taipale L, Sorsa T. Salivary MMP‐8, TIMP‐1, and ICTP as markers of advanced periodontitis. J Clin Periodontol 2010; 37: 487–493. doi: 10.1111/j.1600‐051X.2010.01563.x. Aim: Salivary matrix metalloproteinase (MMP)‐8 and ‐14, tissue inhibitor of matrix metalloproteinase (TIMP)‐1, and pyridinoline cross‐linked carboxyterminal telopeptide of type I collagen (ICTP) were analysed aiming to detect potential markers of advanced periodontitis in saliva. In addition, we compared two MMP‐8 detection methods, a time‐resolved immunofluorometric assay (IFMA) and an enzyme‐linked immunoassay (ELISA), to differentiate periodontitis subjects from controls. Material and Methods: Concentrations of MMP‐8, MMP‐14, TIMP‐1, and ICTP were analysed from salivary specimens of 165 subjects, including 84 subjects having at least 14 teeth with periodontal pocket (pocket depth 4 mm) and 81 subjects without pocket depth as their controls. Results: Salivary MMP‐8 detection by IFMA differentiated periodontitis subjects from controls more strongly than by ELISA. Salivary MMP‐8, TIMP‐1, and ICTP concentrations were higher in periodontitis subjects than those in controls. When only smokers were included in the analysis these differences were lost. The MMP‐8/TIMP‐1 ratio and the combination of MMP‐8 and ICTP differentiated periodontitis and control groups even in smoker subjects. Conclusion: Salivary MMP‐8, TIMP‐1, ICTP, and especially their ratios and combinations are potential candidates in the detection of advanced periodontitis. Differentiating periodontitis and control subjects with salivary MMP‐8 detection is dependent on the selected techniques.