Development Perspective of Bioelectrocatalysis-Based Biosensors

• Published in: Sensors (Basel, Switzerland) Vol. 20; no. 17; p. 4826
• Main Authors: Adachi, Taiki, Kitazumi, Yuki, Shirai, Osamu, Kano, Kenji
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 26.08.2020; MDPI AG
• Subjects: current–potential curve; mass-transfer-controlled amperometric response; multi-enzymatic cascades; multianalyte detection; potentiometric coulometry; Review; potentiometric coulometry; multi-enzymatic cascades; mass-transfer-controlled amperometric response; multianalyte detection; current–potential curve
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s20174826

Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.