SIVQ-LCM Protocol for the ArcturusXT Instrument

• Published in: Journal of visualized experiments no. 89
• Main Authors: Hipp, Jason D., Cheng, Jerome, Hanson, Jeffrey C., Rosenberg, Avi Z., Emmert-Buck, Michael R., Tangrea, Michael A., Balis, Ulysses J.
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 23.07.2014
• Subjects: Algorithms; Animals; Automation - methods; Bioengineering; computer software; Humans; image analysis; Image Processing, Computer-Assisted - methods; Laser Capture Microdissection - instrumentation; Laser Capture Microdissection - methods; Pattern Recognition, Automated; tissues
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/51662

SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.