Zucker Diabetic‐Sprague Dawley Rats Have Impaired Peri‐Implant Bone Formation, Matrix Composition, and Implant Fixation Strength

• Published in: JBMR plus Vol. 7; no. 11; pp. e10819 - n/a
• Main Authors: Anderson, Kyle D, Beckmann, Christian, Heermant, Saskia, Ko, Frank C, Dulion, Bryan, Tarhoni, Imad, Borgia, Jeffrey A, Virdi, Amarjit S, Wimmer, Markus A, Sumner, D Rick, Ross, Ryan D
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.11.2023; Oxford University Press
• Subjects: ANIMAL MODELS; Animals; Apposition; BIOMECHANICS; Bone composition; Bone growth; BONE MATRIX; Cancellous bone; DIABETES; Diabetes mellitus (non-insulin dependent); Epoxy resins; Glucose monitoring; Hyperglycemia; IMPLANTS; Inflammation; Monocyte chemoattractant protein 1; Mutation; Osseointegration; Osteogenesis; Rodents; Sample size; Surgery; Titanium; Transplants & implants; St Louis Missouri; United States > US; IMPLANTS; ANIMAL MODELS; BIOMECHANICS; DIABETES; BONE MATRIX
• ISSN: 2473-4039; 2473-4039
• DOI: 10.1002/jbm4.10819

ABSTRACT An increasing number of patients with type 2 diabetes (T2DM) will require total joint replacement (TJR) in the next decade. T2DM patients are at increased risk for TJR failure, but the mechanisms are not well understood. The current study used the Zucker Diabetic‐Sprague Dawley (ZDSD) rat model of T2DM with Sprague Dawley (SPD) controls to investigate the effects of intramedullary implant placement on osseointegration, peri‐implant bone structure and matrix composition, and fixation strength at 2 and 10 weeks post‐implant placement. Postoperative inflammation was assessed with circulating MCP‐1 and IL‐10 2 days post‐implant placement. In addition to comparing the two groups, stepwise linear regression modeling was performed to determine the relative contribution of glucose, cytokines, bone formation, bone structure, and bone matrix composition on osseointegration and implant fixation strength. ZDSD rats had decreased peri‐implant bone formation and reduced trabecular bone volume per total volume compared with SPD controls. The osseointegrated bone matrix of ZDSD rats had decreased mineral‐to‐matrix and increased crystallinity compared with SPD controls. Osseointegrated bone volume per total volume was not different between the groups, whereas implant fixation was significantly decreased in ZDSD at 2 weeks but not at 10 weeks. A combination of trabecular mineral apposition rate and postoperative MCP‐1 levels explained 55.6% of the variance in osseointegration, whereas cortical thickness, osseointegration mineral apposition rate, and matrix compositional parameters explained 69.2% of the variance in implant fixation strength. The results support the growing recognition that both peri‐implant structure and matrix composition affect implant fixation and suggest that postoperative inflammation may contribute to poor outcomes after TJR surgeries in T2DM patients. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research. In this article, we evaluated the effects of type 2 diabetes on peri‐implant bone using the Zucker Diabetic‐Sprague Dawley (ZDSD) rat and sought to determine the factors that contribute to implant osseointegration and fixation strength. We found that compared to non‐diabetic controls, ZDSD rats had reduced peri‐implant bone formation rates, decreased trabecular bone mass, and altered bone matrix composition. The extent of osseointegration was dependent on bone formation rate and postoperative inflammation, while fixation strength was dependent on cortical structure, formation rates, and matrix composition.