Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Moz...

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Published inToxins Vol. 12; no. 8; p. 500
Main Authors Kuna, Ewelina, Bocian, Aleksandra, Hus, Konrad K., Petrilla, Vladimir, Petrillova, Monika, Legath, Jaroslav, Lewinska, Anna, Wnuk, Maciej
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 04.08.2020
MDPI
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ISSN2072-6651
2072-6651
DOI10.3390/toxins12080500

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Summary:In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three Candida species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of C. albicans and C. tropicalis, affected cell cycle of C. albicans, inhibited C. tropicalis-based biofilm formation and promoted oxidative stress in C. albicans, C. glabrata and C. tropicalis cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three Candida species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of Candida species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate Candida pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments.
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These authors have contributed equally as last authors.
These authors have contributed equally as first authors.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins12080500