Growth, blood health, antioxidant status, immune response and resistance to Aeromonas hydrophila of juvenile yellow catfish exposed to di-2-ethylhexyl phthalate (DEHP)

• Published in: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Vol. 202; pp. 79 - 84
• Main Authors: Yuan, Lixia, Li, Ming, Meng, Fanxing, Gong, Yifu, Qian, Yunxia, Shi, Ge, Wang, Rixin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2017; Elsevier BV
• Subjects: Aeromonas hydrophila; alanine transaminase; Animals; Antioxidant; Antioxidants; Antioxidants - metabolism; aspartate transaminase; blood; blood proteins; body weight; Catfishes; Catfishes - growth & development; DEHP; Diethylhexyl Phthalate; Diethylhexyl Phthalate - toxicity; enzyme activity; fish; Fish Diseases; Fish Diseases - immunology; Fish Diseases - microbiology; glucose; glutathione peroxidase; Gram-Negative Bacterial Infections; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Growth; hepatosomatic index; Immune response; Immunity, Innate; Immunity, Innate - drug effects; immunosuppression; juveniles; malondialdehyde; metabolism; Pelteobagrus fulvidraco; phthalates; specific growth rate; superoxide dismutase; Tachysurus fulvidraco; toxicity; triacylglycerols; Water Pollutants, Chemical; Water Pollutants, Chemical - toxicity; weight gain; Pelteobagrus fulvidraco; Immune response; Antioxidant; Growth; DEHP
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2017.08.004

Triplicate groups of juvenile yellow catfish Pelteobagrus fulvidraco were exposed to three levels of DEHP (0, 0.1 and 0.5mgL−1) for 56days. Fish survival (100%) was not affected by different levels of ambient DEHP. Final body weight, weight gain, specific growth rate and feed intake of fish exposed to 0.5mgL−1 DEHP were the highest. On the contrary, hepatosomatic index of fish exposed to 0.1 and 0.5mgL−1 DEHP were the lowest. Serum total protein, glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase, glucose and triglycerides increased with the increasing concentrations of DEHP exposure. Superoxide dismutase and glutathione peroxidase activities of fish exposed to 0.5mgL−1 DEHP were the lowest, but malondialdehyde contents of fish exposed to 0.1 and 0.5mgL−1 DEHP were higher than that of control fish. Phagocytic indices of the control group were the highest. After being intraperitoneally injected with Aeromonas hydrophila, fish in the control group had the highest expression of toll like receptor 5, and the expression of myeloid differentiation factor 88 of fish exposed to 0.5mgL−1 DEHP was the lowest. This study indicates that DEHP exerts its toxic effects by interfering with hepatic metabolism, inducing ROS generation and malondialdehyde accumulation, leading to blood deterioration and immunosuppression.