On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

• Published in: Metabolites Vol. 10; no. 1; p. 18
• Main Authors: Kambhampati, Shrikaar, Aznar-Moreno, Jose A., Hostetler, Cooper, Caso, Tara, Bailey, Sally R., Hubbard, Allen H., Durrett, Timothy P., Allen, Doug K.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.12.2019; MDPI
• Subjects: Amino acids; Biomass; Carbon; central carbon metabolism; Chromatography; correlations; Developmental stages; fast neutron mutants; Fatty acids; Feeds; Genetic engineering; Genomes; Greenhouses; Humidity; linear models; Lipids; Maturation; Metabolism; Metabolites; Mutation; Nitrogen; oil; Oligosaccharides; protein; Proteins; Raffinose; raffinose family oligosaccharides; seed development; Seeds; Soybeans; Sucrose; United States > US; central carbon metabolism; raffinose family oligosaccharides; fast neutron mutants; oil; protein; seed development; correlations; linear models; soybeans
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo10010018

Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting in tradeoffs for the production of other reserves including oligosaccharides. During the late stages of seed development, the allocation of carbon for storage reserves changes. Lipid and protein levels decline while concentrations of indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since the maternal source of carbon is diminished during seed maturation stages of development, carbon supplied to RFO synthesis likely comes from an internal, turned-over source and may contribute to the reduction in protein and lipid content in mature seeds. In this study, fast neutron (FN) mutagenized soybean populations with deletions in central carbon metabolic genes were examined for trends in oil, protein, sugar, and RFO accumulation leading to an altered final composition. Two lines with concurrent increases in oil and protein, by combined 10%, were identified. A delayed switch in carbon allocation towards RFO biosynthesis resulted in extended lipid accumulation and without compromising protein. Strategies for future soybean improvement using FN resources are described.