Distinguish Dianthus species or varieties based on chloroplast genomes

• Published in: Open life sciences Vol. 18; no. 1; pp. 20220772 - 31
• Main Authors: Meng, Dong, Yang, Liu, Yunlin, Zhao, Guiyan, Yang, Shuwen, Chen, Zhenggang, Xu
• Format: Journal Article
• Language: English
• Published: De Gruyter 28.11.2023
• Subjects: comparative analysis; molecular markers; phylogenetic analysis; simple sequence repeat
• ISSN: 2391-5412; 2391-5412
• DOI: 10.1515/biol-2022-0772

Most plants belonging to the widely distributed genus are used for gardening. Interspecific hybridization of different species leads to blurred genetic backgrounds. To obtain more genomic resources and understand the phylogenetic relationships among species, the chloroplast genomes of 12 species, including nine varieties, were analyzed. The chloroplast genomes of these 12 species exhibited similar sizes (149,474–149,735 bp), with having a chloroplast genome size of 149,604 bp marked by a significant contraction in inverted repeats. In the chloroplast genome of , we identified 124–126 annotated genes, including 83–84 protein-coding genes. Notably, had 83 protein-coding genes but lacked . The repeat sequences of the chloroplast genome were consistent among species, and variations in the sequence were limited and not prominent. However, notable gene replacements were observed in the boundary region. Phylogenetic analysis of indicated that and were most closely related, suggesting that the degree of variation within nine varieties was no less than the variation observed between species. These differences provide a theoretical foundation for a more comprehensive understanding of the diversity within species.