Increased ability of transgenic plants expressing the bacterial enzyme ACC deaminase to accumulate Cd, Co, Cu, Ni, Pb, and Zn

• Published in: Journal of biotechnology Vol. 81; no. 1; pp. 45 - 53
• Main Authors: Grichko, Varvara P, Filby, Brendan, Glick, Bernard R
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 28.07.2000; Amsterdam Elsevier; New York, NY
• Subjects: 1-Aminocyclopropane-1-carboxylic acid; 1-aminocyclopropane-1-carboxylic acid deaminase; ACC deaminase; Bacterial Proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biotechnology; cadmium; Carbon-Carbon Lyases; Carbon-Carbon Lyases - genetics; Carbon-Carbon Lyases - metabolism; cobalt; copper; Environment and pollution; Enzymes; Fundamental and applied biological sciences. Psychology; genetics; Heavy metals; Industrial applications and implications. Economical aspects; lead; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; metabolism; Metals, Heavy; Metals, Heavy - metabolism; Miscellaneous; Mosaic Viruses; Mosaic Viruses - genetics; nickel; Phytoremediation; Plants (botany); Plants, Genetically Modified; Plants, Genetically Modified - metabolism; Promoter Regions, Genetic; Solanum lycopersicum; Solanum lycopersicum var. lycopersicum; Stress; Transgenic tomato; zinc; 1-Aminocyclopropane-1-carboxylic acid; Transgenic tomato; Heavy metals; Stress; ACC deaminase; Phytoremediation; Enzyme; Toxicity; Gene expression; Biological purification; Heavy metal; Pollutant; Enzymatic activity; Vegetable crop; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Transgenic plant; Solanaceae
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/S0168-1656(00)00270-4

Transgenic tomato plants Lycopersicon esculentum (Solanaceae) cv. Heinz 902 expressing the bacterial gene 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, under the transcriptional control of either two tandem 35 S cauliflower mosaic virus promoters (constitutive expression), the rolD promoter from Agrobacterium rhizogenes (root specific expression) or the pathogenesis related PRB-1 b promoter from tobacco, were compared to non-transgenic tomato plants in their ability to grow in the presence of Cd, Co, Cu, Mg, Ni, Pb, or Zn and to accumulate these metals. Parameters that were examined include metal concentration and ACC deaminase activity in both plant shoots and roots; root and shoot development; and leaf chlorophyll content. In general, transgenic tomato plants expressing ACC deaminase, especially those controlled by the PRB-1 b promoter, acquired a greater amount of metal within the plant tissues, and were less subject to the deleterious effects of the metals on plant growth than were non-transgenic plants.