Molecular characterization of Malassezia isolates from dogs using three distinct genetic markers in nuclear DNA

• Published in: Molecular and cellular probes Vol. 21; no. 3; pp. 229 - 238
• Main Authors: Cafarchia, Claudia, Stefania Latrofa, Maria, Testini, Gabriella, Parisi, Antonio, Guillot, Jacques, Gasser, Robin B., Otranto, Domenico
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2007; Elsevier
• Subjects: Animals; Base Sequence; Candida albicans; Cell Nucleus; Chitin Synthase - analysis; Chitin Synthase - genetics; Chitin synthase 2 gene (CHS2); Dermatomycoses - diagnosis; Dermatomycoses - genetics; Dermatomycoses - microbiology; DNA, Fungal - analysis; DNA, Ribosomal - analysis; Dog; Dogs - microbiology; First internal transcribed spacer (ITS-1) of ribosomal DNA; Genetic Markers; Large subunit (LSU) of ribosomal DNA; Life Sciences; Malassezia; Malassezia - genetics; Malassezia - isolation & purification; Malassezia furfur; Malassezia pachydermatis; Molecular characterization; Molecular Sequence Data; Phylogeny; Sequence Homology, Nucleic Acid; Chitin synthase 2 gene (CHS2); Malassezia; Large subunit (LSU) of ribosomal DNA; Molecular characterization; Dog; First internal transcribed spacer (ITS-1) of ribosomal DNA; MOLECULAR CHARACTERIZATION; CHITIN SYNTHASE 2 GENE (CHS2); FIRST INTERNAL TRANSCRIBED SPACER (ITS-1) OF RIBOSOMAL DNA; MALASSEZIA; DOG; LARGE SUBUNIT (LSU) OF RIBOSOMAL DNA
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1016/j.mcp.2007.01.002

Little precise information is available on the systematics, genetics, ecology and epidemiology of yeasts of the genus Malassezia from different animal species. In the present study, one hundred and four isolates of Malassezia (lipid dependent or non-lipid dependent) from dogs were characterized by their chitin synthase 2 gene (CHS2), and the large subunit (LSU) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA sequences, and compared genetically with well-defined reference strains of Malassezia pachydermatis and heterologous species, including Malassezia furfur and Candida albicans. For each locus examined, three main sequence types (i.e. A, B and C) represented all of the 104 isolates, which were designated as genotypes A, B and C, respectively. A fourth, minor sequence type was also defined for the ITS-1. The nucleotide differences among genotypes was consistent with the magnitudes of intraspecific variability reported in previous studies. The genetic analysis of the sequence data sets (for individual loci) showed that all Malassezia genotypes clustered (with moderate to strong support) with the reference sequences of M. pachydermatis to the exclusion of the outgroups M. furfur and C. albicans. The present study reveals that multiple genetic variants of M. pachydermatis occur on dogs. The multilocus approach employed herein provides a foundation for future investigations of M. pachydermatis from other animals and humans, and their ecology and epidemiology.