A rapid electrophoretic method for the detection of serum Lp(a) lipoprotein

• Published in: Clinica Chimica Acta Vol. 185; no. 2; pp. 147 - 155
• Main Authors: Kawakami, Keiko, Tsukada, Atsuko, Okubo, Minoru, Tsukada, Toshihiko, Kobayashi, Tetsuro, Yamada, Nobuhiro, Murase, Toshio
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.11.1989; Elsevier BV; Elsevier
• Subjects: Adult; Age Factors; Aged; Aged, 80 and over; Analytical, structural and metabolic biochemistry; Arteriosclerosis; Arteriosclerosis - blood; Biological and medical sciences; Cerebral Infarction; Cerebral Infarction - blood; Electrophoresis, Agar Gel; Electrophoresis, Agar Gel - methods; Female; Fundamental and applied biological sciences. Psychology; Humans; Lipoprotein electrophoresis; Lipoprotein(a); Lipoproteins; Lipoproteins - blood; Lipoproteins, myelin; Lp(a) lipoprotein; Male; Middle Aged; Myocardial Infarction; Myocardial Infarction - blood; Proteins; Risk Factors; Sex Factors; Myocardial infarction; Cerebral infarction; Lipoprotein electrophoresis; Lp(a) lipoprotein; Heart; Human; Brain; Infarct; Gel electrophoresis; Atherosclerosis; Lipoprotein; Detection
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(89)90037-5

A rapid electrophoretic method detecting serum lipoprotein(a) (Lp(a)) was described. When serum lipoproteins were electrophoresed using an agarose gel film containing 0.6% agarose and 3% sucrose, a distinct extra-band was frequently detected between beta- and prebeta-bands. This lipoprotein band was identified as Lp(a) by immunoblotting technique using anti-Lp(a) serum. Using this electrophoretic method, we studied 1353 subjects with a variety of diseases. The Lp(a)-positive subjects accounted for 8.4% of all. Neither sex nor age influenced the frequency of Lp(a) positivity. Frequencies of definite myocardial infarction in Lp(a)-positive subjects did not differ significantly from those in Lp(a)-negative ones. Those of cerebral infarction in Lp(a)-positive subjects were significantly higher than those in Lp(a)-negative ones. An electrophoretic method described herein is very useful for the rapid detection and screening of Lp(a), and hence for large-scale clinical studies of atherosclerotic risk assessment.