Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota

• Published in: Food science and human wellness Vol. 13; no. 5; pp. 2690 - 2702
• Main Authors: Liu, Qing, Ma, Teng, Feng, Cuijiao, Li, Yalin, Jin, Hao, Shi, Xuan, Kwok, Lai-Yu, Shi, Yan, Chen, Tingtao, Zhang, Heping
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2024; Affiliated Stomatological Hospital of Nanchang University,Nanchang 330000,China; Jiangxi Province Clinical Research Center for Oral Diseases,Nanchang 330000,China%Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010000,China; Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010000,China; The Key Laboratory of Oral Biomedicine of Jiangxi Province; Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010000,China%National Engineering Research Center for Bioengineering Drugs and the Technologies,Institute of Translational Medicine,Nanchang University,Nanchang 330000,China; Department of Endodontics,Affiliated Stomatological Hospital of Nanchang University; Tsinghua University Press
• Subjects: adjuvants; Dental caries; Dental filling; energy; food science; humans; microorganisms; Oral microbiota; Pacific Biosciences (PacBio) single molecule real-time (SMRT) sequencing; Postbiotics; Prevotella; prognosis; Pseudomonas; Salivary microbiota; species diversity; therapeutics; Pacific Biosciences (PacBio) single molecule real-time (SMRT) sequencing; Dental caries; Salivary microbiota; Oral microbiota; Postbiotics; Dental filling; Pacific Biosciences(PacBio)single molecule real-time(SMRT)sequencing
• ISSN: 2213-4530; 2097-0765; 2213-4530
• DOI: 10.26599/FSHW.2022.9250217

Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria. Thus, oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis. We recruited 9 caries-free individuals, and 89 dental caries subjects (5 dropouts). Eighty-nine patients were randomized into three groups: caries (n = 8; no treatment), control (n = 40; filling), and postbiotics (n = 41; filling and 14-day Probio-Eco® intervention). Salivary samples were collected at 0 day (after filling) and 14 days. Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects, and filling could restore a healthier oral microbiota partially and temporarily. Thepostbiotics intervention keeps a low alpha-diversity. Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention. Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly: increased the relative abundance of Pseudomonas and P. reactans, decreased the relative abundance of Prevotella shahii, and enriched the energy metabolism-related pathways. BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention. Collectively, it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries.