Diagnostic Utility of Selected Matrix Metalloproteinases (MMP-2, MMP-3, MMP-11, MMP-26), HE4, CA125 and ROMA Algorithm in Diagnosis of Ovarian Cancer

• Published in: International journal of molecular sciences Vol. 25; no. 11; p. 6265
• Main Authors: Kicman, Aleksandra, Gacuta, Ewa, Kulesza, Monika, Będkowska, Ewa Grażyna, Marecki, Rafał, Klank-Sokołowska, Ewa, Knapp, Paweł, Niczyporuk, Marek, Ławicki, Sławomir
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.06.2024; MDPI
• Subjects: Adult; Aged; Algorithms; Asymptomatic; Biomarkers; Biomarkers, Tumor - blood; CA-125 Antigen - blood; Cancer; Cancer therapies; Case-Control Studies; Chemotherapy; Correlation analysis; Diagnosis; Female; Health aspects; Humans; Matrix Metalloproteinase 11 - blood; Matrix Metalloproteinase 11 - metabolism; Matrix Metalloproteinase 2 - blood; Matrix Metalloproteinase 3 - blood; Matrix Metalloproteinases - blood; Matrix Metalloproteinases - metabolism; Medical prognosis; Medical research; Medicine, Experimental; Membrane Proteins - blood; Membrane Proteins - metabolism; Metastasis; Middle Aged; Ovarian cancer; Ovarian Neoplasms - blood; Ovarian Neoplasms - diagnosis; Proteins - analysis; Proteins - metabolism; ROC Curve; WAP Four-Disulfide Core Domain Protein 2 - analysis; WAP Four-Disulfide Core Domain Protein 2 - metabolism; Women; Womens health; Poland; Taiwan; MMP-2; Serous cystadenomas; ovarian cancer; HE4; MMP-26; MMP-3; CA125; ROMA algorithm; MMP-11; plasma concentration
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25116265

Ovarian cancer (OC) has an unfavorable prognosis. Due to the lack of effective screening tests, new diagnostic methods are being sought to detect OC earlier. The aim of this study was to evaluate the concentration and diagnostic utility of selected matrix metalloproteinases (MMPs) as OC markers in comparison with HE4, CA125 and the ROMA algorithm. The study group consisted of 120 patients with OC; the comparison group consisted of 70 patients with benign lesions and 50 healthy women. MMPs were determined via the ELISA method, HE4 and CA125 by CMIA. Patients with OC had elevated levels of MMP-3 and MMP-11, similar to HE4, CA125 and ROMA values. The highest SE, SP, NPV and PPV values were found for MMP-26, CA125 and ROMA in OC patients. Performing combined analyses of ROMA with selected MMPs increased the values of diagnostic parameters. The topmost diagnostic power of the test was obtained for MMP-26, CA125, HE4 and ROMA and performing combined analyses of MMPs and ROMA enhanced the diagnostic power of the test. The obtained results indicate that the tested MMPs do not show potential as stand-alone OC biomarkers, but can be considered as additional tests to raise the diagnostic utility of the ROMA algorithm.