Interactions of Microcapsules with Human Polymorphonuclear Leucocytes

• Published in: Chemical & pharmaceutical bulletin Vol. 32; no. 7; pp. 2788 - 2794
• Main Authors: MIYAMOTO, MASAKI, SASAKAWA, SHIGERU, KONDO, TAMOTSU, WATANABE, YOSHIHISA
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1984; 公益社団法人日本薬学会; Maruzen; Japan Science and Technology Agency
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Capsules - pharmacology; Chemistry, Pharmaceutical; Humans; In Vitro Techniques; leucocyte interaction; Medical sciences; microcapsules; Neutrophils - metabolism; plasma protein; protein adsorption; Sheep; surface potential; Surface Properties; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Proteins; Blood cell; Blood substitute; Interaction; Granulocyte; Microcapsule; Cell surface
• ISSN: 0009-2363; 1347-5223; 1347-5223
• DOI: 10.1248/cpb.32.2788

Two types of microcapsules with different negative surface potentials, poly (1, 4-piperazinediylterephthaloyl) microcapsules containing various concentrations of sodium polystyrene sulfonate solution and hemolysate-loaded polyamide microcapsules of different degrees of carboxylation, were prepared by using an interfacial polymerization technique, and the interactions of these microcapsules with human polymorphonulcear leucocytes (PMNs) were investigated as a function of the surface potential of the microcapsules in the absence and presence of plasma proteins. The rate of oxygen consumption by PMNs was taken as a measure of the interaction. In the absence of plasma proteins, the rate of oxygen consumption by PMNs increased with increasing difference in surface potential between the microcapsules and PMNs. The presence of plasma proteins affected the rate of oxygen consumption, which was dependent on the protein species present and related to the surface potential of the microcapsules. This was interpreted as showing that the surface potential of the microcapsules exerts a great influence on the mode and amount of protein adsorption on the microcapsule surface.