Evaluation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 toxicity on Neuro-2a, NG108-15 and MCF-7 cell lines
•We have established the toxicity equivalency for three lipophilic toxins on three cell lines.•We have found unexpected differences between toxins related to cell-lines.•We have determined the best cell model for DTXs toxins detection.•We have used certified reference toxins for data standardization...
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| Published in | Toxicology in vitro Vol. 29; no. 1; pp. 59 - 62 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.02.2015
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0887-2333 1879-3177 1879-3177 |
| DOI | 10.1016/j.tiv.2014.09.002 |
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| Abstract | •We have established the toxicity equivalency for three lipophilic toxins on three cell lines.•We have found unexpected differences between toxins related to cell-lines.•We have determined the best cell model for DTXs toxins detection.•We have used certified reference toxins for data standardization.
Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines. |
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| AbstractList | Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines. •We have established the toxicity equivalency for three lipophilic toxins on three cell lines.•We have found unexpected differences between toxins related to cell-lines.•We have determined the best cell model for DTXs toxins detection.•We have used certified reference toxins for data standardization. Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines. Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines.Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines. |
| Author | Sureda, Francesc X. Soliño, Lucia Diogène, Jorge |
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| Keywords | Okadaic acid (OA) Dinophysistoxin-1 (DTX-1) Cell-based assay (CBA) Marine toxins Dinophysistoxin-2 (DTX-2) |
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| Snippet | •We have established the toxicity equivalency for three lipophilic toxins on three cell lines.•We have found unexpected differences between toxins related to... Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human... |
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| SubjectTerms | Cell Line Cell-based assay (CBA) Dinophysistoxin-1 (DTX-1) Dinophysistoxin-2 (DTX-2) Dose-Response Relationship, Drug Humans Marine toxins Marine Toxins - toxicity MCF-7 Cells - drug effects Okadaic acid (OA) Okadaic Acid - toxicity Pyrans - toxicity |
| Title | Evaluation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 toxicity on Neuro-2a, NG108-15 and MCF-7 cell lines |
| URI | https://dx.doi.org/10.1016/j.tiv.2014.09.002 https://www.ncbi.nlm.nih.gov/pubmed/25238672 https://www.proquest.com/docview/1629588745 https://www.proquest.com/docview/1647022683 |
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