Evaluation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 toxicity on Neuro-2a, NG108-15 and MCF-7 cell lines

• Published in: Toxicology in vitro Vol. 29; no. 1; pp. 59 - 62
• Main Authors: Soliño, Lucia, Sureda, Francesc X., Diogène, Jorge
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2015
• Subjects: Cell Line; Cell-based assay (CBA); Dinophysistoxin-1 (DTX-1); Dinophysistoxin-2 (DTX-2); Dose-Response Relationship, Drug; Humans; Marine toxins; Marine Toxins - toxicity; MCF-7 Cells - drug effects; Okadaic acid (OA); Okadaic Acid - toxicity; Pyrans - toxicity; Okadaic acid (OA); Dinophysistoxin-1 (DTX-1); Cell-based assay (CBA); Marine toxins; Dinophysistoxin-2 (DTX-2)
• ISSN: 0887-2333; 1879-3177; 1879-3177
• DOI: 10.1016/j.tiv.2014.09.002

•We have established the toxicity equivalency for three lipophilic toxins on three cell lines.•We have found unexpected differences between toxins related to cell-lines.•We have determined the best cell model for DTXs toxins detection.•We have used certified reference toxins for data standardization. Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines.