Major Gene Identification and Quantitative Trait Locus Mapping for Yield-Related Traits in Upland Cotton (Gossypium hirsutum L.)

• Published in: Journal of Integrative Agriculture Vol. 13; no. 2; pp. 299 - 309
• Main Authors: XIA, Zhe, ZHANG, Xin, LIU, Yang-yang, JIA, Zi-fang, ZHAO, Hai-hong, LI, Cheng-qi, WANG, Qing-lian
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2014; Science Press; Elsevier
• Subjects: genetic improvement; Gossypium hirsutum; lint cotton; lint yield; loci; major gene; major gene segregation analysis QTL mapping yield-related traits upland cotton; major genes; marker-assisted selection; phenotypic variation; QTL mapping; quantitative trait loci; seed yield; segregation analysis; upland cotton; yield-related traits; major gene; segregation analysis; upland cotton; yield-related traits; QTL mapping
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(13)60508-0

Segregation analysis of the mixed genetic model of major gene plus polygene was used to identify the major genes for cotton yield-related traits using six generations P1, P2, F1, B1, B2, and F2 generated from the cross of Baimian 1 × TM-1. In addition to boll size and seed index, the major genes for the other five traits were detected: one each for seed yield, lint percentage, boll number, lint index; and two for lint yield. Quantitative trait locus/loci (QTL) mapping was performed in the F2 and F2:3 populations of above cross through molecular marker technology, and a total of 50 QTL (26 suggestive and 24 significant) for yield-related traits were detected. Four common QTL were discovered: qLP-3b(F2)/qLP-3(F2:3) and qLP-19b (F2)/qLP-19(F2:3) for lint percentage, qBN-17(F2)/qBN-17(F2:3) for boll number, and qBS-26b(F2)/qBS-26(F2:3) for boll size. Especially, qLP-3b(F2)/qLP-3(F2:3), not only had LOD scores <3 but also exceeded the permutation threshold (5.13 and 5.29, respectively), correspondingly explaining 23.47 and 29.55% of phenotypic variation. This QTL should be considered preferentially in marker assisted selection (MAS). Segregation analysis and QTL mapping could mutually complement and verify, which provides a theoretical basis for genetic improvement of cotton yield-related traits by using major genes (QTL).