Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling

• Published in: Current Issues in Molecular Biology Vol. 47; no. 7; p. 539
• Main Authors: Shieh, Ju-Sheng, Chin, Yu-Tang, Yeh, Tsu-Te, Guo, Jiong Jiong, Chang, Fung-Wei, Cheng, Hui-Rong, Hsu, Hung-Han, Huang, Wei-Lun, Huang, Han-Hsiang, Hsieh, Ya-Yu, Chiang, Chien-Ping, Wang, Shih-Ching
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.07.2025; MDPI
• Subjects: avian mesenchymal stem cells; Bio-Pulsed priming; Clinical trials; Collagen; extracellular vesicles; hair follicle activation; Health aspects; MicroRNA; regenerative dermatology; RNA sequencing; Skin; skin rejuvenation; Stem cells; United States; Taiwan; clinical trial; extracellular vesicles; avian mesenchymal stem cells; non-invasive therapy; collagen biosynthesis; hair follicle activation; microRNA profiling; Bio-Pulsed priming; skin rejuvenation; regenerative dermatology
• ISSN: 1467-3045; 1467-3037; 1467-3045
• DOI: 10.3390/cimb47070539

Small extracellular vesicles (sEVs) derived from mesenchymal stem cells have emerged as promising therapeutic agents in regenerative dermatology. This study evaluated the safety and efficacy of Bio-Pulsed avian mesenchymal stem cell-derived sEVs (AMSC-sEVs), topically applied for hair follicle stimulation and skin rejuvenation. Two prospective, single-arm clinical trials were conducted: one involving 30 participants using a hair ampoule over 60 days, and the other involving 30 participants applying a facial essence for 28 days. Objective measurements demonstrated significant improvements in the anagen/telogen hair ratio, reduced shedding, increased collagen density, and reduced wrinkle depth and pigmentation. Small RNA sequencing and qPCR profiling confirmed that Bio-Pulsed AMSC-sEVs were enriched with regenerative microRNAs, such as miR-21-5p and miR-199a-5p, associated with anti-inflammatory and anti-aging effects. No adverse events were reported. These findings suggest that Bio-Pulsed AMSC-sEVs may offer a safe, non-invasive, and cell-free approach to enhance skin and hair regeneration in human subjects.