Detection of canine parvovirus type 2c by a commercially available in-house rapid test

Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 2...

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Published inThe veterinary journal (1997) Vol. 184; no. 3; pp. 373 - 375
Main Authors Decaro, Nicola, Desario, Costantina, Beall, Melissa J., Cavalli, Alessandra, Campolo, Marco, DiMarco, Anthony A., Amorisco, Francesca, Colaianni, Maria Loredana, Buonavoglia, Canio
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.06.2010
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ISSN1090-0233
1532-2971
1532-2971
DOI10.1016/j.tvjl.2009.04.006

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Abstract Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >10 5 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
AbstractList Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >10 5 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >105 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
Author Campolo, Marco
Buonavoglia, Canio
Amorisco, Francesca
Colaianni, Maria Loredana
Desario, Costantina
DiMarco, Anthony A.
Decaro, Nicola
Cavalli, Alessandra
Beall, Melissa J.
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Keywords In-house assay
Canine parvovirus
Diagnosis
New variant canine parvovirus 2c
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Snippet Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect...
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SubjectTerms analysis
Animals
Canine parvovirus
Carnivore protoparvovirus 1
classification
Diagnosis
disease detection
DNA, Viral
DNA, Viral - analysis
dog diseases
Dog Diseases - diagnosis
Dog Diseases - virology
Dogs
Feces
Feces - virology
Feline panleukopenia virus
In-house assay
isolation & purification
methods
microbial detection
New variant canine parvovirus 2c
Parvoviridae Infections
Parvoviridae Infections - diagnosis
Parvoviridae Infections - veterinary
Parvoviridae Infections - virology
Parvovirus, Canine
Parvovirus, Canine - classification
Parvovirus, Canine - isolation & purification
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
rapid methods
Sensitivity and Specificity
test sensitivity
Time Factors
veterinary
viral diseases of animals and humans
virology
Title Detection of canine parvovirus type 2c by a commercially available in-house rapid test
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https://www.ncbi.nlm.nih.gov/pubmed/19410488
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