Detection of canine parvovirus type 2c by a commercially available in-house rapid test
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 2...
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Published in | The veterinary journal (1997) Vol. 184; no. 3; pp. 373 - 375 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.06.2010
|
Subjects | |
Online Access | Get full text |
ISSN | 1090-0233 1532-2971 1532-2971 |
DOI | 10.1016/j.tvjl.2009.04.006 |
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Abstract | Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a,
n
=
51; CPV-2b,
n
=
50; CPV-2c,
n
=
100), containing CPV DNA loads >10
5 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. |
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AbstractList | Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a,
n
=
51; CPV-2b,
n
=
50; CPV-2c,
n
=
100), containing CPV DNA loads >10
5 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >105 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. |
Author | Campolo, Marco Buonavoglia, Canio Amorisco, Francesca Colaianni, Maria Loredana Desario, Costantina DiMarco, Anthony A. Decaro, Nicola Cavalli, Alessandra Beall, Melissa J. |
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Keywords | In-house assay Canine parvovirus Diagnosis New variant canine parvovirus 2c |
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References | Decaro, Desario, Elia, Martella, Mari, Lavazza, Nardi, Buonavoglia (bib5) 2008; 31 Decaro, N., Cirone, F., Desario, C., Elia, G., Lorusso, E., Colaianni, M.L., Martella, V., Buonavoglia, C., in press. Severe parvovirosis in a repeatedly vaccinated 12-year-old dog. Veterinary Record. Buonavoglia, Martella, Pratelli, Tempesta, Cavalli, Buonavoglia, Bozzo, Elia, Decaro, Carmichael (bib1) 2001; 82 Decaro, Elia, Martella, Campolo, Desario, Camero, Cirone, Lorusso, Lucente, Narcisi, Scalia, Buonavoglia (bib3) 2006; 133 Decaro, Desario, Parisi, Martella, Lorusso, Miccolupo, Mari, Colaianni, Cavalli, Di Trani, Buonavoglia (bib6) 2009; 385 Desario, Decaro, Campolo, Cavalli, Cirone, Elia, Martella, Lorusso, Camero, Buonavoglia (bib8) 2005; 121 Decaro, Elia, Martella, Desario, Campolo, Di Trani, Tarsitano, Tempesta, Buonavoglia (bib2) 2005; 105 Decaro, Desario, Addie, Martella, Vieira, Elia, Zicola, Davis, Thompson, Thiry, Truyen, Buonavoglia (bib4) 2007; 13 Kapil, Cooper, Lamm, Murray, Rezabek, Johnston, Campbell, Johnson (bib9) 2007; 45 Truyen (bib10) 2006; 117 Decaro (10.1016/j.tvjl.2009.04.006_bib6) 2009; 385 10.1016/j.tvjl.2009.04.006_bib7 Truyen (10.1016/j.tvjl.2009.04.006_bib10) 2006; 117 Desario (10.1016/j.tvjl.2009.04.006_bib8) 2005; 121 Buonavoglia (10.1016/j.tvjl.2009.04.006_bib1) 2001; 82 Kapil (10.1016/j.tvjl.2009.04.006_bib9) 2007; 45 Decaro (10.1016/j.tvjl.2009.04.006_bib2) 2005; 105 Decaro (10.1016/j.tvjl.2009.04.006_bib3) 2006; 133 Decaro (10.1016/j.tvjl.2009.04.006_bib4) 2007; 13 Decaro (10.1016/j.tvjl.2009.04.006_bib5) 2008; 31 |
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SubjectTerms | analysis Animals Canine parvovirus Carnivore protoparvovirus 1 classification Diagnosis disease detection DNA, Viral DNA, Viral - analysis dog diseases Dog Diseases - diagnosis Dog Diseases - virology Dogs Feces Feces - virology Feline panleukopenia virus In-house assay isolation & purification methods microbial detection New variant canine parvovirus 2c Parvoviridae Infections Parvoviridae Infections - diagnosis Parvoviridae Infections - veterinary Parvoviridae Infections - virology Parvovirus, Canine Parvovirus, Canine - classification Parvovirus, Canine - isolation & purification polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary rapid methods Sensitivity and Specificity test sensitivity Time Factors veterinary viral diseases of animals and humans virology |
Title | Detection of canine parvovirus type 2c by a commercially available in-house rapid test |
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