Detection of canine parvovirus type 2c by a commercially available in-house rapid test

• Published in: The veterinary journal (1997) Vol. 184; no. 3; pp. 373 - 375
• Main Authors: Decaro, Nicola, Desario, Costantina, Beall, Melissa J., Cavalli, Alessandra, Campolo, Marco, DiMarco, Anthony A., Amorisco, Francesca, Colaianni, Maria Loredana, Buonavoglia, Canio
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2010
• Subjects: analysis; Animals; Canine parvovirus; Carnivore protoparvovirus 1; classification; Diagnosis; disease detection; DNA, Viral; DNA, Viral - analysis; dog diseases; Dog Diseases - diagnosis; Dog Diseases - virology; Dogs; Feces; Feces - virology; Feline panleukopenia virus; In-house assay; isolation & purification; methods; microbial detection; New variant canine parvovirus 2c; Parvoviridae Infections; Parvoviridae Infections - diagnosis; Parvoviridae Infections - veterinary; Parvoviridae Infections - virology; Parvovirus, Canine; Parvovirus, Canine - classification; Parvovirus, Canine - isolation & purification; polymerase chain reaction; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; rapid methods; Sensitivity and Specificity; test sensitivity; Time Factors; veterinary; viral diseases of animals and humans; virology; In-house assay; Canine parvovirus; Diagnosis; New variant canine parvovirus 2c
• ISSN: 1090-0233; 1532-2971; 1532-2971
• DOI: 10.1016/j.tvjl.2009.04.006

Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >10 5 DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.