In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

• Published in: Nanomaterials (Basel, Switzerland) Vol. 8; no. 7; p. 539
• Main Authors: Shaheen, Fozia, Aziz, Muhammad, Fatima, Mahvish, Khan, Muhammad, Ahmed, Faisal, Ahmad, Riaz, Ahmad, Muhammad, Alkhuraiji, Turki, Akram, Muhammad, Raza, Rizwan, Ali, Syed
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.07.2018; MDPI
• Subjects: Apoptosis; Biocompatibility; Biomedical materials; Breast cancer; cellular morphology; Chemical properties; Cytotoxicity; Emission analysis; Field emission microscopy; Graphene; graphene oxide (GO); Malignancy; Morphology; Nanocomposites; Nanomaterials; Nanotechnology; Organic chemistry; Raman spectroscopy; Reactive oxygen species; reactive oxygen species (ROS); Scanning electron microscopy; Science; Singlet oxygen; Spectroscopic analysis; Statistical analysis; Studies; Toxicity; Ultraviolet spectroscopy; X ray analysis; X ray spectra; X-ray diffraction; Zinc oxide; Saudi Arabia; Lahore Pakistan; Pakistan; Riyadh Saudi Arabia; China; reactive oxygen species (ROS); cytotoxicity; cellular morphology; graphene oxide (GO)
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano8070539

Graphene-based materials have attracted considerable interest owing to their distinctive characteristics, such as their biocompatibility in terms of both their physical and intrinsic chemical properties. The use of nanomaterials with graphene as a biocompatible agent has increased due to an uptick in dedication from biomedical investigators. Here, GO-ZnO was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray analysis (EDAX), and Raman spectroscopy for structural, morphological, and elemental analysis. The toxic extent of GO-ZnO was noted by a methyl-thiazole-tetrazolium (MTT), while cellular morphology was observed towards the MCF-7 cells using an inverted microscope at magnification 40×. The cytotoxic effect of GO-ZnO investigated the cell viability reduction in a dose-dependent manner, as well as prompted the cell demise/destruction in an apoptotic way. Moreover, statistical analysis was performed on the experimental outcomes, with p-values < 0.05 kept as significant to elucidate the results. The generation of reactive oxygen species (ROS) demonstrated the potential applicability of graphene in tumor treatment. These key results attest to the efficacy of GO-ZnO nanocomposites as a substantial candidate for breast malignancy treatment.