Occurrence and genetic diversity of CRESS DNA viruses in wild birds: a Hungarian study

• Published in: Scientific reports Vol. 10; no. 1; p. 7036
• Main Authors: Kaszab, Eszter, Lengyel, György, Marton, Szilvia, Dán, Ádám, Bányai, Krisztián, Fehér, Enikő
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.04.2020; Nature Publishing Group
• Subjects: 45/23; 45/77; 631/208/325/2483; 631/326/596; Animals; Animals, Wild - genetics; Aquatic birds; Birds - virology; Conserved sequence; Deoxyribonucleic acid; DNA; DNA viruses; DNA Viruses - classification; DNA Viruses - genetics; Evolution; Genetic diversity; Genetic Variation; Genomes; Humanities and Social Sciences; Hungary; multidisciplinary; Nucleotide sequence; Phylogeny; Recombination; Science; Science (multidisciplinary); Species; Viruses; Whole genome sequencing; Hungary
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-63795-x

Circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (CRESS) DNA viruses have been detected in a variety of animal taxa. In this study, cloacal swab samples (n = 90) were examined for CRESS DNA viruses from 31 wild bird species living at various aquatic sites in Hungary to identify possible reservoirs of viruses pathogenic to domestic poultry. A total of 30 (33.3%) specimens tested positive with pan-CRESS DNA virus specific PCR. Goose circovirus (GoCV), Duck associated cyclovirus 1 (DuACyV-1) and Garrulus glandarius associated circular virus 1 (GgaCV-1) were detected in nine, three and two different bird species, respectively. Selected specimens were subjected to whole genome sequencing. The obtained sequence data revealed conserved gene structure within the identified virus species and detected homologous (within GoCV) and possible heterologous recombination (within DuACyV-1) events. Results presented here provide new information on the genomic diversity and evolution of selected CRESS DNA viruses.