Identification of polyphenols and their metabolites in human urine after cranberry-syrup consumption

• Published in: Food and chemical toxicology Vol. 55; pp. 484 - 492
• Main Authors: Iswaldi, Ihsan, Arráez-Román, David, Gómez-Caravaca, Ana María, Contreras, María del Mar, Uberos, José, Segura-Carretero, Antonio, Fernández-Gutiérrez, Alberto
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.05.2013; Elsevier
• Subjects: Adult; Biological and medical sciences; Chromatography, Liquid; computer software; cranberries; Cranberry; Female; glucose; Humans; ingestion; isomers; isotopes; Male; Mass Spectrometry; Medical sciences; metabolites; myricetin; Polyphenols; Polyphenols - analysis; Polyphenols - urine; quercetin; scopoletin; solid phase extraction; syrups; Toxicology; Ultra-performance liquid chromatography; Urinary metabolites; urine; Vaccinium macrocarpon; Polyphenols; Urinary metabolites; Mass spectrometry; Ultra-performance liquid chromatography; Cranberry; Consumption; Human; Urine; Ultra performance liquid chromatography; Biological fluid; Metabolite; Identification; Excreta; Syrup; Polyphenol; Plant origin; Phenols; Physical method; Organic compounds
• ISSN: 0278-6915; 1873-6351; 1873-6351
• DOI: 10.1016/j.fct.2013.01.039

► Human urinary metabolites of polyphenols have been profiled using UPLC-Q-TOF MS. ► Prior to the analysis, metabolites were extracted using an optimised SPE procedure. ► 32 Metabolites (phase I and II) were tentatively identified. As the beneficial effects of American cranberry (Vaccinium macrocarpon) can be partly attributed to its phenolic composition, the evaluation of the physiological behaviour of this fraction is crucial. A rapid and sensitive method by ultra-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been used to identify phenolic metabolites in human urine after a single dose of cranberry syrup. Prior to the analysis, metabolites were extracted using an optimised solid-phase extraction procedure. All possible metabolites were investigated based on retention time, accurate mass data and isotope and fragmentation patterns. Free coumaroyl hexose (isomer 1 and 2), dihydroxybenzoic acid, caffeoyl glucose, dihydroferulic acid 4-O-β-d-glucuronide, methoxyquercetin 3-O-galactoside, scopoletin, myricetin and quercetin, together with other 23 phase-I and phase-II metabolites, including various isomers, could be tentatively identified in the urine. Afterwards, the metabolites were simultaneously screened in the urine of different subjects at 0, 2, 4, and 6h after the ingestion of cranberry syrup by Target AnalysisTM software.