Change Profiles in Matrix Metalloproteinase-2 and -9 in Induced Endometriosis in Mice

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 2; pp. 188 - 192
• Main Author: 陈琼华 邱娜璇 濮德敏 周玉明 李天 杨宏毅
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2010; Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xiamen Medical University, Xiamen 361004, China%Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xiamen Medical University, Xiamen 361004, China%Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Endometriosis - enzymology; Endometriosis - etiology; Female; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Medicine; Medicine & Public Health; Mice; Mice, Inbred BALB C; MMP; Peritoneal Diseases - enzymology; Peritoneal Diseases - etiology; RNA, Messenger - genetics; RNA, Messenger - metabolism; 基质金属蛋白酶-2; 子宫内膜异位症; 聚合酶链反应; 蛋白质水平; 酶联免疫吸附试验; endometriosis; animal model; matrix metalloproteinase
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-010-0211-4

To examine the changes in matrix metalloproteinase-2 （MMP-2） and -9 （MMP-9） in the development and progression of endometriosis, real time quantitative polymerase chain reaction, enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21^st day after the induction in mice with induced endometriosis （experimental group） and sham-operated animals （controls）. The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group. MMP-9 had two or three peaks during the 21 days, taking place at day 1, 4 and 15. It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.