Associations Between Autoimmune Thyroid Disease Prognosis and Functional Polymorphisms of Susceptibility Genes, CTLA4, PTPN22, CD40, FCRL3, and ZFAT, Previously Revealed in Genome-wide Association Studies

• Published in: Journal of clinical immunology Vol. 32; no. 6; pp. 1243 - 1252
• Main Authors: Inoue, Naoya, Watanabe, Mikio, Yamada, Hiroya, Takemura, Kazuya, Hayashi, Fumiaki, Yamakawa, Noriko, Akahane, Maiko, Shimizuishi, Yu, Hidaka, Yoh, Iwatani, Yoshinori
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.12.2012; Springer Nature B.V
• Subjects: Adult; Alleles; Antibodies; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; CD40 Antigens - genetics; CTLA-4 Antigen - genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Genome-Wide Association Study; Genotype; Graves Disease - diagnosis; Graves Disease - genetics; Hashimoto Disease - diagnosis; Hashimoto Disease - genetics; Humans; Immunology; Infectious Diseases; Internal Medicine; Male; Medical Microbiology; Middle Aged; Polymorphism, Restriction Fragment Length; Prognosis; Protein Tyrosine Phosphatase, Non-Receptor Type 22 - genetics; Receptors, Immunologic - genetics; Severity of Illness Index; Transcription Factors - genetics; severity; Hashimoto’s disease; polymorphism; GWAS; intractability; Graves’ disease
• ISSN: 0271-9142; 1573-2592; 1573-2592
• DOI: 10.1007/s10875-012-9721-0

Purpose Genome-wide association studies have revealed several susceptibility genes among patients with autoimmune thyroid disease (AITD), including CTLA4 , PTPN22 , FCRL3 , and ZFAT . However, any possible association between these genes and AITD prognosis remains unknown. The objective of this study was to identify associations between polymorphisms of these genes and AITD prognosis. Methods We genotyped functional polymorphisms, including CTLA4 CT60, CTLA4 +49A/G, CTLA4 -1147C/T, CTLA4 -318C/T, PTPN22 -1123C/G, PTPN22 SNP37, CD40 -1C/T, FCRL3 -169C/T, ZFAT Ex9b-SNP10, and ZFAT Ex9b-SNP2, in 197 AITD patients carefully selected from 456 registered AITD patients, and 86 control subjects. The restriction fragment length polymorphism method was used for genotyping. Results The CD40 -1CC genotype and C allele were significantly more frequent in patients with Graves’ disease (GD) in remission than in those with intractable GD ( P  = 0.041 and P  = 0.031, respectively). The FCRL3 -169TT genotype was significantly less frequent in patients with intractable GD than in those with GD in remission ( P  = 0.0324). For a ZFAT Ex9b-SNP10 polymorphism, the TT genotype and T allele were significantly more frequent in patients with severe Hashimoto’s disease (HD) than in those with mild HD ( P  = 0.0029 and P  = 0.0049, respectively). For a CTLA4 CT60 polymorphism, the antithyrotropin receptor antibody levels at the onset of GD were significantly higher in those with the GG genotype than in those with other genotypes ( P  = 0.0117). Conclusions CD40 and FCRL3 gene polymorphisms were associated with GD intractability, and ZFAT polymorphism was associated with HD severity but not its development.