A1 receptor mediated adenosinergic regulation of perifornical–lateral hypothalamic area neurons in freely behaving rats

• Published in: Neuroscience Vol. 167; no. 1; pp. 40 - 48
• Main Authors: Rai, S, Kumar, S, Alam, M.A, Szymusiak, R, McGinty, D, Alam, M.N
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 28.04.2010
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; Adenosine A1 Receptor Agonists; Adenosine A1 Receptor Antagonists; Animals; Biological and medical sciences; Catheterization; Central Nervous System Agents - pharmacology; Electrodes, Implanted; Fundamental and applied biological sciences. Psychology; Hypothalamus - drug effects; Hypothalamus - physiology; Intracellular Signaling Peptides and Proteins - metabolism; Light; Male; Microdialysis; Microelectrodes; Neurology; Neurons - drug effects; Neurons - physiology; Neuropeptides - metabolism; Orexins; Photoperiod; Proto-Oncogene Proteins c-fos - metabolism; Rats; Rats, Sprague-Dawley; Receptor, Adenosine A1 - metabolism; Sleep - drug effects; Sleep - physiology; Sleep. Vigilance; Vertebrates: nervous system and sense organs; Wakefulness - drug effects; Wakefulness - physiology; Xanthines - pharmacology; NonREM; posterior–lateral hypothalamus; melanin-concentrating hormone; electromyogram; sleep; perifornical–lateral hypothalamus; orexin; perifornical–lateral hypothalamic area; HCRT; PF-LHA; adenosine A 1 receptor; CPDX; EMG; N 6-cyclopentyladenosine, an A 1 receptor agonist; TBS; EEG; non-rapid eye movement sleep; Fos-IR; artificial cerebrospinal fluid; electroencephalogram; adenosine; MCH; tris buffered saline; CPA; aCSF; hypocretin; c-fos protein immunoreactivity; 1,3-dipropyl-8-phenylxanthine, an A 1 receptor antagonist; Posterior hypothalamus; Adenosine; Rat; Rodentia; Central nervous system; A1 Adenosine receptor; Lateral hypothalamus; Neuropeptide; Encephalon; Vertebrata; Mammalia; Neuron; Sleep; receptor; adenosine A; Animal; perifornical-lateral hypothalamus; posterior-lateral hypothalamus; Orexin; Biological receptor
• ISSN: 0306-4522; 1873-7544; 1873-7544
• DOI: 10.1016/j.neuroscience.2010.01.044

Abstract The perifornical–lateral hypothalamic area (PF-LHA) plays a central role in the regulation of behavioral arousal. The PF-LHA contains several neuronal types including wake-active hypocretin (HCRT) neurons that have been implicated in the promotion and/or maintenance of behavioral arousal. Adenosine is an endogenous sleep factor and recent evidence suggests that activation and blockade of adenosine A1 receptors within the PF-LHA promote and suppress sleep, respectively. Although, an in vitro study indicates that adenosine inhibits HCRT neurons via A1 receptor, the in vivo effects of A1 receptor mediated adenosinergic transmission on PF-LHA neurons including HCRT neurons are not known. First, we determined the effects of N6 -cyclopentyladenosine (CPA), an adenosine A1 receptor agonist, on the sleep–wake discharge activity of the PF-LHA neurons recorded via microwires placed adjacent to the microdialysis probe used for its delivery. Second, we determined the effects of CPA and that of an A1 receptor antagonist, 1,3-dipropyl-8-phenylxanthine (CPDX) into the PF-LHA on cFos-protein immunoreactivity (Fos-IR) in HCRT and non-HCRT neurons around the microdialysis probe used for their delivery. The effect of CPA on Fos-IR was studied in rats that were kept awake during lights-off phase, whereas the effect of CPDX was examined in undisturbed rats during lights-on phase. CPA significantly suppressed the sleep–wake discharge activity of PF-LHA neurons. Doses of CPA (50 μM) and CPDX (50 μM) that suppressed and induced arousal, respectively, in our earlier study [Alam MN, Kumar S, Rai S, Methippara M, Szymusiak R, McGinty D (2009) Brain Res 1304:96–104], significantly suppressed and increased Fos-IR in HCRT and non-HCRT neurons. These findings suggest that wake-promoting PF-LHA system is subject to increased endogenous adenosinergic inhibition and that adenosine acting via A1 receptors, in part, inhibits HCRT neurons to promote sleep.