Modular Breath Analyzer (MBA): Introduction of a Breath Analyzer Platform Based on an Innovative and Unique, Modular eNose Concept for Breath Diagnostics and Utilization of Calibration Transfer Methods in Breath Analysis Studies

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 12; p. 3776
• Main Authors: Jaeschke, Carsten, Padilla, Marta, Glöckler, Johannes, Polaka, Inese, Leja, Martins, Veliks, Viktors, Mitrovics, Jan, Leja, Marcis, Mizaikoff, Boris
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 21.06.2021; MDPI
• Subjects: breath analysis; Calibration; calibration transfer; Cancer; Chromatography; Costs; Disease; Gases; low sensing chamber volume; Machine learning; Mass spectrometry; Medical research; Metabolism; MOX sensors; piecewise direct standardization; Scientific imaging; Sensors; standard samples; Standardization; VOCs; Volatile organic compounds
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26123776

Exhaled breath analysis for early disease detection may provide a convenient method for painless and non-invasive diagnosis. In this work, a novel, compact and easy-to-use breath analyzer platform with a modular sensing chamber and direct breath sampling unit is presented. The developed analyzer system comprises a compact, low volume, temperature-controlled sensing chamber in three modules that can host any type of resistive gas sensor arrays. Furthermore, in this study three modular breath analyzers are explicitly tested for reproducibility in a real-life breath analysis experiment with several calibration transfer (CT) techniques using transfer samples from the experiment. The experiment consists of classifying breath samples from 15 subjects before and after eating a specific meal using three instruments. We investigate the possibility to transfer calibration models across instruments using transfer samples from the experiment under study, since representative samples of human breath at some conditions are difficult to simulate in a laboratory. For example, exhaled breath from subjects suffering from a disease for which the biomarkers are mostly unknown. Results show that many transfer samples of all the classes under study (in our case meal/no meal) are needed, although some CT methods present reasonably good results with only one class.