A Pectin-Rich, Baobab Fruit Pulp Powder Exerts Prebiotic Potential on the Human Gut Microbiome In Vitro

• Published in: Microorganisms (Basel) Vol. 9; no. 9; p. 1981
• Main Authors: Foltz, Martin, Zahradnik, Alicia Christin, Van den Abbeele, Pieter, Ghyselinck, Jonas, Marzorati, Massimo
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 17.09.2021; MDPI
• Subjects: absorption; acetates; Acetic acid; Acidification; Adansonia digitata; adults; Bacteria; Bacteroidetes; baobab fruit pulp powder; Bifidobacterium; butyrates; Chain branching; Colon; Composition; Dialysis; Dietary fiber; Digestive system; digestive tract; Fatty acids; Feces; Fermentation; fruit pulp; Fruits; Gastrointestinal system; Gastrointestinal tract; Glucose; gut microbiota; Hemodialysis; human health; Human subjects; humans; in vitro; interindividual variation; Intestinal microflora; intestinal microorganisms; Inulin; Lactic acid; Lactobacilli; Lactobacillus; Metabolites; Microbiomes; Microbiota; Microorganisms; Pectin; polygalacturonic acid; Polysaccharides; Powder; prebiotic; Prebiotics; Propionic acid; Pulp; Saccharides; Sugar; Belgium; Germany
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9091981

Increasing insight into the impact of the gut microbiota on human health has sustained the development of novel prebiotic ingredients. This exploratory study evaluated the prebiotic potential of baobab fruit pulp powder, which consists of pectic polysaccharides with unique composition as compared to other dietary sources, given that it is rich in low methoxylated homogalacturonan (HG). After applying dialysis procedures to remove simple sugars from the product (simulating their absorption along the upper gastrointestinal tract), 48 h fecal batch incubations were performed. Baobab fruit pulp powder boosted colonic acidification across three simulated human adult donors due to the significant stimulation of health-related metabolites acetate (+18.4 mM at 48 h), propionate (+5.5 mM at 48 h), and to a lesser extent butyrate (0.9 mM at 48 h). Further, there was a trend of increased lactate levels (+2.7 mM at 6h) and reduced branched chain fatty acid (bCFA) levels (−0.4 mM at 48 h). While Bacteroidetes levels increased for all donors, donor-dependent increases in Bifidobacteria, Lactobacilli, and Firmicutes were observed, stressing the potential interindividual differences in microbial composition modulation upon Baobab fruit pulp powder treatment. Overall, Baobab fruit pulp powder fermentation displayed features of selective utilization by host microorganisms and, thus, has promising prebiotic potential (also in comparison with the ‘gold standard’ prebiotic inulin). Further research will be required to better characterize this prebiotic potential, accounting for the interindividual differences, while aiming to unravel the potential resulting health benefits.