Protocol for sorting and culturing of mouse early erythroid progenitor BFU-E cells

• Published in: STAR protocols Vol. 5; no. 1; p. 102718
• Main Authors: Li, Yao, Liang, Ziyu, Wang, Hailin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.03.2024; Elsevier
• Subjects: Animals; Cell Biology; Cell culture; Cell Culture Techniques; Cell isolation; Cell separation/fractionation; Erythroid Precursor Cells; Erythropoiesis; Flow Cytometry; Mice; Cell separation/fractionation; Cell culture; Flow Cytometry; Cell isolation; Cell Biology
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2023.102718

Techniques allowing the long-term culture of the burst-forming unit of erythroid (BFU-E) progenitor cells are essential for understanding erythropoiesis. Here, we present a protocol for sorting mouse BFU-E cells and culturing them in a medium that promotes BFU-E cell expansion. We describe steps for isolating BFU-E cells from mouse fetal livers by combining magnetic microbeads with flow cytometry and culturing BFU-E cells with a specific expansion media. This approach can enhance the production of BFU-E cells. For complete details on the use and execution of this protocol, please refer to Li et al..1 [Display omitted] •Isolation of mouse BFU-E cells from fetal liver•Steps for magnetic bead-based sorting followed by flow cytometry•Expansion of BFU-E cells in specific expansion medium Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Techniques allowing the long-term culture of the burst-forming unit of erythroid (BFU-E) progenitor cells are essential for understanding erythropoiesis. Here, we present a protocol for sorting mouse BFU-E cells and culturing them in a medium that promotes BFU-E cell expansion. We describe steps for isolating BFU-E cells from mouse fetal livers by combining magnetic microbeads with flow cytometry and culturing BFU-E cells with a specific expansion media. This approach can enhance the production of BFU-E cells.