Fabrication of a paper-based analytical device for multiple colorimetric analysis via inkjet-printing and paper-cutting

• Published in: Biochip journal Vol. 9; no. 2; pp. 139 - 143
• Main Authors: Yu, Jun Ho, Jeong, Seong-Gun, Lee, Chang-Soo, Hwang, Jun-Young, Kang, Kyung-Tae, Kang, Heuiseok, Lee, Sang-Ho
• Format: Journal Article
• Language: English
• Published: Seoul The Korean BioChip Society (KBCS) 01.06.2015; Springer Nature B.V; 한국바이오칩학회
• Subjects: Biomedical Engineering and Bioengineering; Biotechnology; Bovine serum albumin; Channels; Chemistry; Chemistry and Materials Science; Colorimetry; Hydrophobicity; Inkjet printing; Original Article; Polyvinyl chloride; Printing; Serum albumin; 생물공학; Printing; Cutting; Inkjet; Paper Chip; Colorimetric analysis
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-015-9207-2

We introduce a process for the fabrication of a paper-based analytical device (PAD) for multiple colorimetric analysis via paper- cutting and inkjet-printing. To create the selective flow channels, hydrophobic barriers were formed by using hydrophobic polyvinyl chloride tape-cutting and ultraviolet-curable resin printing. Multiple paper channels were isolated by the hydrophobic barriers on a single chip. An assay model was created from bovine serum albumin (BSA) and tetrabromophenol blue (TBPB) to verify the feasibility of the fabricated PAD. A multiple colorimetric analysis was demonstrated a reaction with different BSA concentrations after the TBPB regent was elaborately deposited onto the circular test spot areas by inkjet printing.