Simultaneous saccharification and fermentation of Eastern redcedar heartwood and sapwood using a novel size reduction technique

• Published in: Bioresource technology Vol. 161; pp. 1 - 9
• Main Authors: Ramachandriya, Karthikeyan D., Wilkins, Mark, Pardo-Planas, Oscar, Atiyeh, Hasan K., Dunford, Nurhan T., Hiziroglu, Salim
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.2014; Elsevier
• Subjects: Acid bisulfite pretreatment; Biofuels; Biological and medical sciences; Biotechnology; delignification; Drying; Eastern redcedar; Ethanol; Ethanol - metabolism; ethanol fermentation; Ethyl alcohol; Fermentation; Fundamental and applied biological sciences. Psychology; glucans; Glucans - metabolism; Grounds; Heartwood; Juniperus; Juniperus virginiana; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Particle Size; Saccharification; Saccharomyces cerevisiae; Sapwood; Size reduction; SSF; Sulfites; Wood; Wood - chemistry; Wood - metabolism; Sapwood; Acid bisulfite pretreatment; Heartwood; SSF; Eastern redcedar; Acids; Saccharification; Pretreatment; Fermentation
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.005

[Display omitted] •Effect of particle size and wood zone was determined on glucan-to-ethanol yield.•Size reduction from 2mm sieve size crumbles® to fines was unnecessary.•Sapwood produced 13% greater ethanol yield than heartwood.•Highest wood glucan-to-ethanol yield of 95% was obtained with sapwood crumbles®.•Wood zone should be considered as a quality variable for ethanol production. This study investigated the effect of two wood zones (sapwood versus heartwood) and size reduction techniques [Crumbles® (Crumbles® is a registered trademark of Forest Concepts, LLC, Auburn, WA, USA) particles versus ground particles] on wood glucan-to-ethanol yield after acid bisulfite pretreatment and simultaneous saccharification and fermentation (SSF) of Eastern redcedar. SSFs were conducted at 8% solids loading (w/w dry basis) using Accellerase® 1500 at a loading of 46FPU/g glucan and Saccharomyces cerevisiae D5A for ethanol fermentation. The size reduction technique had no effect on ethanol yield. However, sapwood glucan-to-ethanol yields were significantly greater than heartwood yields. The highest wood glucan-to-ethanol yield of 187L/dryMg (95% of theoretical) was achieved with sapwood crumbled particles in 240h. Ground sapwood, crumbled heartwood and ground heartwood achieved ethanol yields of 89%, 81% and 80% of theoretical in 240h, respectively. Preliminary mass balances showed 100% glucan recovery with crumbled sapwood and extensive (72%) delignification.